THE TOBACCO BEETLE. Al 
The amount of material varied from a few ounces of tobacco up to 
20 pounds of loose tobacco or tobacco refuse, and the time of treat- 
ment varied from 14 hours to 30 days. Exposures of over 5 days, 
in all experiments, gave satisfactory results, the tobacco being com- 
pletely sterilized. <A bale of infested cigar tobacco kept in cold stor- 
age for 28 days under the conditions mentioned above at a tempera- 
ture of approximately 14° F. was found to have been completely 
freed from all stages of the beetle. 
EFFECT OF COLD STORAGE ON EGGS OF THE TOBACCO BEETLE. 
A large number of separate experiments were made with eggs of 
the tobacco beetle to determine the effect of low temperatures in cold 
storage. In these experiments eggs were placed in cigars and exposed 
at temperatures ranging from 12° to 20° F., the length of exposure 
varying from 24 hours to 16 days. All boxes of cigars were wrapped 
_ with paper. After removal from cold storage the material was placed 
in an incubator and kept at a constant temperature of 86° F. with 
humidity from 80 to 90 per cent. The checks were kept in the same 
incubator until the period of incubation had passed. In these experi- 
ments none of the eggs exposed to cold hatched, while hatching of 
the check lots was normal. 
In another series eggs on leaf tobacco or in cells on microscope 
slides were exposed. Exposures to cold were made without special 
protection such as ordinarily is afforded by the food substance of the 
insect. Other conditions of the experiments were practically the 
same as described in the preceding series. The time of treatment 
ranged from 5 hours to 7 days, with temperatures varying from 14° 
to 18° F. In most experiments in which the time of exposures was 
shorter than 24 hours, the temperature was constant at 14° F. Ex- 
posures of less than 24 hours did not give satisfactory results, as all 
or part of the eggs hatched in most of the experiments. In experi- 
ments in which the duration of treatment was more than 24 hours 
all eggs were killed. 
EFFECT OF REFRIGERATION ON QUALITY OF MANUFACTURED TOBACCO. 
In order to determine whether or not cold storage seriously in- 
jures manufactured tobacco, cigars, cigarettes, and smoking and 
chewing tobacco were placed in cold storage for periods ranging 
from 30 to 50 days. The stock used for the experiment was fresh 
and in good condition. An exact duplicate of each brand was kept 
for the same period, in perfect condition, in the humidor or storage 
room at a cigar store. Part of the material put in cold storage was 
in sealed metal containers and part merely wrapped with paper in 
