SACBROOD. 



43 



four volumes of water to one of ripened honey to obtain the strength 

 recommended. The honey solution by volume, therefore, is about 

 20 per cent honey. 



A suspension of the virus of sacbrood in such a solution is dis- 

 tributed in test tubes placed in an empty hive body and allowed to 

 ferment at outdoor temperature. After periods reckoned in days 

 colonies are inoculated as was done in case of the sugar solutions 

 described above. The following results were obtained from the 

 experiments performed: 



Table X. — Resistance of sacbrood virus to fermentative processes in a 20 per cent honey 

 solution at outdoor temperature. 



Date of inoculation. 



Sept. 11, 1915 

 Sept. 13, 1915 

 Sept. 14, 1915. 

 Aus.4, 1915.. 

 Sept. 15, 1915 

 Sept. 14, 1915 

 Sept. 22, 1915. 

 Sept. 17, 1915 

 Sept. 8, 1915.. 



Results of inoculation. 



Sacbrood produced. 



Do. 

 No disease produced. 



Do. 



Do. 



Do. 



Do. 



Do. 



Do. 



In the presence of fermentative processes taking place in a 20 per 

 cent honey solution at outdoor temperature it will be observed that 

 the virus of sacbrood in the experiments recorded in Table X was 

 destroyed in six days. The outdoor temperature during these 

 experiments was quite warm. Had it been cooler, the time for the 

 destruction of the virus would have been somewhat increased. In 

 the making of vinegar it may be concluded that the virus of sacbrood, 

 should it be present in the honey used, would be destroyed in a com- 

 paratively short time as a result of fermentation. 



RESISTANCE OF SACBROOD VIRUS TO THE PRESENCE OF PUTREFACTIVE 



PROCESSES. 



Larvae containing the virus of sacbrood are crushed and suspended 

 in water. A small quantity of soil is added. The suspension is 

 strained and distributed in test tubes. These are allowed to stand at 

 room temperature in a state of putrefaction. After periods reckoned 

 in days colonies free from the disease are inoculated, each with the 

 contents of a single tube added to sirup. From experiments of this 

 kind the results following have been obtained. 



