SACBROOD. 45 



Table XII. — Resistance of sacbrood virus to carbolic acid — Continued. 



Date of inoculation. 



Strength 



of car- 

 bolic acid 

 used. 



Time in 

 suspen- 

 sion. 



Results of inoculation. 



Sept. 3, 1914. 

 Sept. 18, 1914 

 June 23, 1915. 

 Sept. 17, 1915 

 Aug. 12, 1915 

 Aug. 21, 1915. 



June 23, 1915. 

 July 1,1915.. 



June 23, 1915. 

 Aug. 12, 1915 



Per cent. 

 2 

 2 

 2 

 2 

 2 

 2 



Days. 

 1 



16 

 25 

 38 

 42 

 50 



Hours. 

 3 



7 



Days. 



Sacbrood produced. 



Do. 



Do. 

 No disease produced. 



Do. 



Do. 



Sacbrood produced. 

 Do. 



No disease produced. 

 Do. 



From the preliminary results recorded in Table XII it will be 

 observed that the virus of sacbrood shows a marked resistance to the 

 disinfecting power of carbolic acid. Under the conditions of the 

 experiments the virus resisted its action for more than three weeks 

 in J, 1, and 2 per cent aqueous solutions. 



These results lead naturally to a consideration of the effect of 

 drugs on the virus of sacbrood in the treatment of the disease. On 

 this point complete data are yet wanting. 



While the disinfecting power of a compound, as shown in experi- 

 ments such as those described above for carbolic acid, may indicate 

 something as to the value of the compound as a drug, it does not 

 necessarily prove its value. More definite proof is gained through 

 feeding colonies with the virus suspended in honey medicated with 

 the drug, and then continuing to feed the inoculated colonies with 

 honey similarly medicated daily thereafter until the time for the 

 appearance of the disease. 



To illustrate the nature of experiments which are being conducted 

 to determine the value of drugs in the treatment of sacbrood, experi- 

 ments with quinine and carbolic acid are here referred to. A colony 

 was fed the virus of sacbrood suspended in honey and water, equal 

 parts, to which was added 5 grains of the bisulphate of quinine to 

 one-half pint of diluted honey, and on each of the five days following 

 the inoculation the same colony was fed diluted honey containing no 

 virus, but medicated with quinine in the same way. On the seventh 

 day following the inoculation with the virus there was found to be a 

 large quantity of sacbrood produced in the colony so inoculated and 

 treated. 



A similar experiment in which carbolized honey was used gave 

 like results. These experiments, although not furnishing conclusive 

 proof, do indicate something of what might be expected from the 

 use of quinine or carbolic acid as a drug in the treatment of sacbrood. 



