26 BULLETIN 405, U. S. DEPARTMENT OF AGRICULTURE. 
These extracts were made by Mr. O. F. Black, chemical biologist 
in the Office of Drug-Plant and Poisonous-Plant Investigations, and — 
were used in Be injections upon mice. Table 2 gives 
the results. 
TABLE 2.—Kelative toxicity to mice of the various parts of Lupinus leucopsis. 
| | 
Animal. | Plant used. | 
| Extract i 
Part of plant. used. Per 25 Result. 
Mouse. Weight. Weight. | grams of 
animal. 
Grams CC Grams Grams. | 
No7 ae 24 WieaVeSctEL 2.8 Sas o i! 12, 1.25 | Death in 7.5 minutes. 
No. 35--- -=-% - 2655 ele ea Qe er Sane aa adn .9 . 833 | Death in 9.5 minutes. 
No. 445722. 23 55h |e ARE OG 452 08 5238 eke 45, 6 .64 | Death in 26 minutes. 
ING Agios AAR ences Ose eens eee ails -18 . 322 | Not sick. 
Infepsieessees 26.5 | Pods (seed removed) ae -6 .565 | Death in 4.5 minutes. 
INOS3 fase D0 eilteser ae bak Pcs 2 eee ee - 25 .3 .375 | Deathin 6.5 minutes. 
Nps 452 =4o55- 19 =e ni dothe |e sees ms 18 . 237 | Not sick. 
INQIS8 2425-2 15.5 ods eed shed) === >= oak <o .482 | Very sick; recovery. 
MNos43 222 22 IQSS is32e- Os 32> See eee .3 36 457 | Sick; recovery. 
NOs«MbO ee NON ie A Oa eae eee .35 .42 552 | Deathin 11 minutes. 
NoI39 Ss 24 aaa cists collection) 22 . 24 -25 | Death in 12.5 minutes. 
INOSA0S 2235-2 19a ES OOl eee ere eee -15 - 18 . 237 | Sick; recovery. 
INOsAI 25 122. - 22.5 | Seed Lists collection) LZ . 24 .266 | Not sick. 
IN ONED Eres DAs ASS OR as ae pene a ae -20 .3 .357 | Death in 14 minutes. 
Table 2 shows that the amount necessary to kill a mouse on the 
basis of the standard weight of 25 grams is approximately as follows: 
Grams. 
TG OAV CBE see 2 a ec ae eB ee tener 0. 6 to 0. 64 
iPods (seed removed) os = * 522. 25 kien ee ees eee as eFC gees 1s 
Pods(seed shed). oe orcs Se eic te ee ee eae a .5 
Seeds. coligetion OL AOI 3s. i eee es ee eee SAFI a 
Seeds, collection: of Vue eee eee oe a a ee een ae . 25 
Inasmuch as it is known from other experiments that the toxic and 
lethal doses are practically the same, it is fair to assume that this 
table gives, approximately, not only the lethal dose but also the 
toxic dose. From Table 2 it appears that the seeds collected in 
1914 were slightly more toxic than those collected in the preceding 
year. Possibly a certain amount of the toxicity was lost in keeping. 
It appears also that the pods from which the seed had been shed 
were only about half as toxic as the seed. The pods from which the 
seed had been removed were considerably more toxic than the pods 
from which the seed had been shed, and the leaves were only about 
one-half to one-third as toxic as the seed. These figures do not con- 
form very closely to those obtained in the field experiments upon 
sheep, but perhaps as closely as could be expected under the differ- 
ent conditions of experimentation. An especially interesting feature 
of this experiment was the definite proof obtained by Mr. Black by 
analytical methods of the presence of the alkaloids in the leaves, 
with the consequent deduction, confirmed by actual experiments, 
