LUPINES AS POISONOUS PLANTS. 95 
staggered when she attempted to walk. At 11.22 a. m. she stood 
swaying from side to side. At 11.45 a. m. she was lying upon her 
belly with her head on one side, her respiration being about 200. At 
11.50 a. m. she was up on her feet and commenced to butt into the 
fence again. She soon fell and lay panting. Her respiration was 
160. At 11.57 a. m. she was lying upon one side and panting. At 
12 noon she suddenly got upon her feet and started butting the 
corners, but immediately fell over, holding her head on one side. 
The chewing motions, as noted before, were marked. At 12.15 p. m. 
she got upon her feet, tried to run but fell over; she picked herself 
up, however, and started to butt into the corners. At 12.17 p. m. 
she fell down and lay quietly. At 12.30 p. m. she was on her side 
struggling in an attack of dyspnea. At this time she was bled by 
cutting the angular artery of the eye. At 12.35 p. m. her respiration 
| was 160. At 12.40 p. m. the movements of the jaws and lips still 
continued. At 1.10 p. m. she was struggling and the muscles of the 
flanks were trembling. Her pulse was so fast that it was impossible 
to count it. Her temperature was 107.6° F. At 1.11 p. m. she had 
fallen over on her side and was moving her body about. At 1.13 p.m. 
her respiration was barely perceptible, and at 1.15 p. m. it stopped. 
The movements of respiration and of the heart stopped at about the 
same time. , 
In the autopsy petechiz were found upon the auricles of the heart. 
The lungs were congested. The walls of the ileum were injected, with 
some ecchymoses. ‘The mesenteric blood vessels were injected. 
LABORATORY EXPERIMENTS WITH EXTRACTS OF LUPINE SEED UPON 
MICE. 
Preliminary experiments showed that mice are very susceptible to 
poisoning by extracts of lupine seed injected intraperitoneally. 
Accordingly, a few experiments were undertaken to determine by 
this method the relative toxicity of extracts of various parts of the 
lupine plant. Seeds, pods, and leaves of Lwpinus leucopsis collected 
at Greycliff, Mont., in the summers of 1913 and 1914 were used for 
these experiments. The following is a description of the method 
used in preparing the extracts: 
Material was taken from each part of the plant which it was desired to examine, 
and all the samples were ground in the same mill set to the same degree of fineness. 
They were all ground the same day and at approximately the same time. From each 
form of material a sample of 20 grams was taken, placed in a flask, and macerated 
with 150 c. c. of Prolius’s solution for 48 hours. The maceration of the different 
samples examined was begun and ended at the same time. Ninety c. c. of Prolius’s 
extract was then taken from each flask, filtered, and evaporated down spontaneously. 
The residue of each was then extracted with 1 per cent of HCl, using 3 portions. 
The solution was filtered off, neutralized with Na,CO,, and made up to 10 c. c., so 
that 1 c. c. of the solution was equivalent to 1.2 grams of the sample. 
