56 BULLETIN 380, U, S. DEPARTMENT OF AGRICULTURE. 



diameter, which agreed in every respect with cankers produced on 

 varieties of Japanese chestnuts in this country (PL XXII). 



Other specimens in this collection showed well-developed perithecia 

 and ascospores. The ascospore measurements made at the time, as 

 well as the cultures of the Chinese fungus and the inoculation experi- 

 ments on O. dentata, are described in the previous paper by the 

 writers (76, p. 296). 



Shortly after this first series of inoculations was made subcul- 

 tures of the Chinese material were sent to several investigators who 

 had been studying the chestnut-bark disease, in order that the 

 Endothia from China might be tested as soon as possible under 

 American conditions by inoculations at various points throughout 

 the known range of the disease. 



A series of inoculations was made by Prof. J. Franklin Collins at 

 Martic Forge, Pa., on July 14, 1913, using American and grafted 

 Paragon and grafted Japanese chestnut trees. Another series of 

 inoculations, 56 in number, was made at the same locality Septem- 

 ber 10, 1913, by Dr. Caroline Eumbold on grafted Paragon chest- 

 nuts. Twenty inoculations were made on native chestnuts at Ander- 

 son, Pa., October 2, 1913, by Dr. F. D. Heald and R. A. Studhalter. 

 Inoculations with the Chinese Endothia were made at Leesburg, Va., 

 on both Castanea dentata and C. pumila by G. Flippo Gravatt and 

 J. T. Rogers, August 16, 1913. 



All these investigators made duplicate inoculations with American 

 material, and all agreed that the Chinese fungus was identical in its 

 effects on the host with the American chestnut-blight fungus. Dur- 

 ing the season of 1914 numerous inoculations with material from 

 China were made by the writers at various points in New Hamp- 

 shire, Massachusetts, Connecticut, New York, Delaware, and Mary- 

 land, while others have been made in Rhode Island by Prof. Collins 

 with the same results. 



ADDITIONAL CHINESE SPECIMENS. 



Since the publication of the previous paper (76) additional speci- 

 mens of E. parasitica from China have been received from Meyer; 

 one collected at Changli, Chihli Province, China, October 13, 1913, 

 by Mrs. Mary S. Clemens ; a quantity of material collected by Meyer 

 himself in the village of Tachingko, near Taianfu, Shantung, China, 

 March 21, 1914; and another collected by him at Yatyeko, Shensi, 

 China, September 2, 1914. A few cankers have also been sent by 

 Meyer, collected by him at Shihbonshan, near Hangchow, Chekiang 

 Province, China, June 26, 1915. The label on this specimen bears 

 the further comment, "very destructive in this locality." Cultures 

 have been made from all these specimens and have invariably proved 

 to be identical with cultures of E. parasitica found in this country. 



