64 



BULLETIN 380, U. S. DEPARTMENT OF AGRICULTURE. 



and Vitis sp. Of these, Acer rubrum and Carya glabra gave numerous small 

 pycnidia. 



On July 10 the following hosts were inoculated at Woodstock, N. Y., with 

 E. fluens from Europe: Acer rubrum, A. pervnsylvanicum, Carya ovata, Corylus 

 americana, Fraxinus americana, Hamamelis virginiana, Kalmia latifolia, Popu- 

 his grandidentata, Prunus serotina, Rhus glabra, Salia? sp., Sassafras variifo- 

 lium, and Syringa vulgaris. Each host was inoculated in six or seven places, 

 but all failed to develop except two inoculations on Acer pennsylvanicum and 

 one on Corylus americana. 



The results cited above are so largely negative that they prove very 

 little except that the European strain shows no special affinity for 

 these hosts in America. 



ENDOTHIA FLUENS MISSISSIPPIENSIS. 



Only five collections of Endothia flu-ens mississippiensis have thus 

 far been made, three on Castanea dentata and two on Quercus sp. 

 From the results of the inoculations its host relations appear very 

 similar to those of E. fluens. The results are; shown in Table V. 



Table V. — Inoculations icith Endothia fluens mississippiensis on Castanea and 



Quercus. 



Source of culture. 



Host inoculated. 



Date. 



Number 

 of inocu- 

 lations. 



Number 

 showing 

 pycnidia. 



Castanea 



Castanea 



Jan. 20,1912 

 May 8, 1913 

 do 



8 

 4 

 4 

 9 

 12 

 12 



8 



Do 



do 



4 



Do 



do 



4 



Do 



Quercus prinus 



do 





Do 



Castanea 



Apr. 18,1914 

 do 



10 



Quercus 



do 



10 











The inoculations of January 20, 1912, showed no signs of growth 

 until early in May, when the first signs of pycnidium formation were 

 observed. The inoculations with Endothia fluens mississippiensis 

 made May 8, 1913, showed within three weeks discolored areas near 

 the cut which were larger than those about the check cuts. On July 

 25, 1913, all of the inoculations of E. fluens mississippiensis marked 

 "successful" showed the beginnings of pycnidium formation. By 

 August 30, 1913, they were producing pycnospores, which when cul- 

 tured proved to be E. fluens mississippiensis. 



Inoculations were made in April, 1914, for the purpose of com- 

 paring the material collected on oak with that collected on chest- 

 nut. No difference was detected, and there was no indication of active 

 parasitism. This form behaved in this respect exactly as did the E. 

 fluens from Virginia both on Castanea dentata and Quercus prinus. 



A series of inoculations parallel to that made with E. fluens was 

 made with E. fluens mississippiensis. The same hosts were used, 

 and in most cases the dates and places of the inoculation were the 

 same. The results of all that showed any growth are given in 

 Table VI. 



