68 



BULLETIN 380, U. S. DEPARTMENT OF AGRICULTURE. 



22 in this locality on Acer rubrum, Gary a glabra, Comics fiorida, 

 Fagus grandifolia, Liriodendron tulipifera, Quercus prinus, Sas- 

 safras variifolium, Vaccinium sp., and Vitis sp. without success. 

 On April 18, the following hosts were inoculated in Virginia: Acer 

 rubrum, Betula nigra, Benzoin aestivale, Carpinus caroliniana, Carya 

 glabra, Cornus ftorida, Fagus grandifolia, Liriodendron tulipifera, r 

 Primus serotina, Quercus alba, Vlraus americana, and Vitis sp. 

 Each host was inoculated in from four to six places. Of these, 

 pycnidia were produced only on Acer rubrum, Carpinus, and Lirio- 

 dendron. A similar series was made on the same hosts in the same 

 place on May 27. Inoculations on one tree of Quercus alba showed 

 undoubted evidence of parasitism and is described below. 



On July 9 and 11 from five to fourteen inoculations were made 

 on each of the following hosts at Woodstock, N. Y. : Acer rubrum, 

 Betula alba, Carya ovata, Fagus grandifolia, Fraxinus americana, 

 Hamamelis virginiana, Juglans cinerea, Kolmia latifolia, Nyssa syl- 

 vatica, Ostrya virginiana, Populus grandidentata, Prunus serotina, 

 Rhus typhina, Quercus rubra, Salix sp., Samhucus canadensis, and 

 Sassafras variifolium. Pycnidia appeared on Acer rubrum and 

 Ostrya only. The fungus made considerable growth on two plants 

 of Rhus typhina, partly girdling branches one-half inch in diameter 

 and producing distinct fans. The fans were, however, much smaller 

 than those usually found in Castanea. Inoculations were made at 

 Avon, Conn., July 15, on Acer saccharum, Betula alba, Carya glabra, 

 Cornus fiorida, and Ostrya virginiana. Pycnidia developed only 

 on Ostrya. The successful inoculations with Endothia parasitica 

 are shown in Table VII. 



Table VII. — Successful inoculations in 1914 with Endothia parasitica on Jiosts 



other than Castanea. 



Locality. 



Date. 



Host. 



Number 

 ofinocu- 

 lations. 



Number 

 success- 

 ful.! 





Apr. 18 

 ...do 



Acer rubrum 



9 

 6 

 6 



4 

 14 



6 

 15 



7 



Do 



Carpinus caroliniana 



2 



Do 



...do. . 





1 



Do 



May 27 

 July 11 

 ...do 





4 





Acer pennsylvanicum 



4 



Do 





2 





July 15 



do 



4 











1 Inoculations producing pycnidia are classed as successful. 



It must be noted that while pycnidia were produced in the cases 

 listed as successful, there was no indication of parasitism, nor did 

 the growth extend beyond the tissue injured by the cut except in 

 Quercus and Ehus. 



Out of about 400 inoculations with Endothia parasitica on hosts 

 other than Castanea, about TO of which were made on different 



