\ 
24 BULLETIN 810, U. S. DEPARTMENT OF AGRICULTURE. 
each with the contents of a single flask. A summary of the experi- 
ments is contained in Table XIII: 
TABLE XIII.—Resistance of Bacillus pluton in honey at room temperature 
Date ofinoculation. | Periodin honey. Results of inoculation. 
| Months. | Days. 
1, AO ie C54 ae eee a eer Ree Nhe (ARM Sea 4/E , 
ee iss ee Oe eae | on pee foulbrood produced. 
SEER Do EGE ee ee SS 1 6 Do. 
TP URETS ren EE ee eas Ae Bigs Oey Ln, Voy eee | 1 11 Do. 
IS 2S AGL PRE PRE ON Ras oo eee 1 15 Do. 
Rugs, 19 2 esky SA ee eel 1 17 | Do. 
Prrby Lo AO sae ee en ee De Clea 1 25 | Do 
1g) (eral 1 Se PUR On ata Oem Oe BD YF 2 7 | Do. 
Sept lO 10th 25 oo ee es | 2 25 | Do. 
Aug. ee PO Ie Pe Oe as RR fey, OSES = SD 3 0 | Do. 
May (91916. oe aoe ee Re eee eee 7 0 | No disease produ 
Msi ya 1G fas uc Se ae see ae ee a 7 17 | Do. aes oad 
than Ope Ot 5 ose ew SL ee. ee 8 0 Do. 
USES Syd C1 Pea a ee a PF aa i ah 8 0 Do. 
eyed pO ps nots ate Sok chet Bb Se mer ba Oe pen 8 25 Do. 
Ney 12c19iy 52 TORS eA ae Se 8 26 Do. 
pS Foes See aS ee ee oe ee eee 9 5 Do. 
icp RISE eee oe ek ge eee 9 5 Do. 
w 11 Sse (2 (0 ee a cae ee ok Rd 9 11 Do. 
Mea byt ONG one rk pot oes rea Pace 10 6 | Do. 
Experimental evidence recorded in Table XIII shows that the 
virus of European foulbrood when suspended in honey at room tem- 
perature ceased to be virulent in from 3 to 7 months. 
VIABILITY OF BACILLUS PLUTON IN POLLEN 
Preliminary experiments were made to determine the viability of 
Bacillus pluton in pollen. Pollen is removed from brood-comb, and 
an aqueous suspension of the organism obtained from the stomachs 
of larve sick or recently dead of the disease is added to it until a 
moderately thick, pastelike mass is obtained. This is distributed in 
Petri dishes and allowed to stand at room and refrigerator tempera- 
tures, respectively. After different intervals of time the contents of 
a single dish, after being suspended in water, are added to about 
300 c. c. of sirup and the suspension is fed to a colony, using the 
indirect method. The results show that Bacillus pluton was viru- 
lent after 7 months at room temperature and for more than 10 
months in the refrigerator. The maximum period during which the 
organism will remain alive in these two environments has not been 
determined. 
RESISTANCE OF BACILLUS PLUTON TO CARBOLIC ACID 
Preliminary experiments were made to determine the effect of 
carbolic acid on the virus of European foulbrood. An aqueous sus- 
pension of the contents of the stomachs of larve sick or dead of the 
disease is first made. A measured quantity of this suspension is 
added to an equal quantity of an aqueous suspension of carbolic acid 
