~ 
> «eee 
EUROPEAN FOULBROOD. i 
tively. In Tables XI and XII. which follow. are summarized the 
experiments performed: 
TABLE XI.—Bacillus pluton in the presence of putrefactive processes at incubator 
temperature 
Period of 
Date ofinoculation. putre- Results of inoculation. 
| faction. 
Days. 
MAM ORDO OIG en soe oa nite ote isa telela bats Sao Mane cle's 9 | European foulbrood produced. 
Sept. 2, 1916.....-.--- noaecrogeueEr Sausee soos sosubSeseemnes 7 | No disease produced. 
INE IB ee Ree Se eo SSG aA osc aes oeors Saertomesoered 8 Do. 
Sys ie OIE Oke beeeae ooudoc eee comecserore asato 7oeSUEDne 13a ase DO: 
AOU a NOT eA AIS SA Set oe oar esate Sam Nees See se 15 Do. 
Sept. 10, 1915..... otal i ed AR at a Das Soe A LS ia 16 Do. 
Tubes WIG oe OS ook bbesete as So bebeos SosoeenosssuseuanasdsAde | 18 Do. 
JN (ys BV MI Shee 5 eGe> a6 eddie os sesoddedecu dae coseodeceosece | 19 Do. 
BNE OA PLO ie eres ee Nate apie Naas 3 8 Nese ayia Recor ata iat 26 Do. 
TasLeE XII.—Bacillus pluton in the presence of putrefactive processes at room 
temperature 
Period of 
Date ofinoculation. ae Results of inoculation. 
ion. 
Days 
2 Ue EM Eh eS ae adocpcadanccoanc eH seer ene oradad aoce 8 | European foulbrood produced. 
ulya le lOl sete nr cans Prac aaat ove ee ee fener eee 10 Do. 
VIKA eee oe Ce Sse RR eee atieaco iG ser oo Ses oo ona Ese Reon 14 Do. 
AE re GA MS ah ste) Sek iE pe ares a le a Pe 16 Do. 
WRI OE Ee es a bebe SE oa Succes Cae Seas Sense nh Seer 18 Do. 
Sop tee qlOla ae setae sonia ete a ole ee teke ei Meiaes wae wee BEE wmee 18 Do. 
INTER OB Oy See Os Re hae Ae ea dbe oie OoSSoe eS ansoee aa 18 Do. 
Sey O Ror WING) are ea a ee ee Se ose needs 26 Do. 
PICS SLO Wo nse ee oc n apelena)s comes as eens faae.ces 27 Do. 
INTTSR IS LONG ee eee tts ee cla Noes Rae Senecio Ue ple alee cise einleloe 21 | No disease produced. 
ST Hy TRIES ee SOR ee eB eee Se aot ae Soe ees GegSaa- esupone 35 | Do. 
SEOs HO Ne Soe won Choo otenedabsedd SoouscneoeseuesedeeeeRar 51 | Do. 
HATA SEP LOU He cieteier =P stnete Brae ctia ere aiste ee cic[ats e/a oe eters Ces ea 52 | Do. 
As shown by Tables XI and XII Bacillus pluton is destroyed in 
the presence of putrefactive processes. At incubator temperature 
it resisted the effects of these processes for from 7 to 13 days and 
at room temperature for from 21 to 35 days. 
During August and September, 1916, preliminary experiments 
were made testing the resistance of Bacillus pluton to putrefaction 
at outdoor temperature. The parasite was alive and virulent after 
40 days. The maximum period during which it will remain so has 
not been determined. 
VIABILITY OF BACILLUS PLUTON IN HONEY 
Honey suspensions of Bacillus pluton from the stomach contents 
of larvee sick or recently dead of European foulbrood were made and 
distributed in flasks each containing about 300 c. c. These were 
allowed to stand at room temperature shielded from the light. At 
intervals thereafter colonies free from the disease were inoculated 
