~ 
22 BULLETIN 810, U. S. DEPARTMENT OF AGRICULTURE. 
rose) solution. A bit of soil was added to inoculate it further. 
Records were made on suspensions fermenting at incubator and room 
temperatures, respectively. Tables TX and X which follow sum- 
marize experiments made: 
’ TaBLe 1X.—Bacillus pluton in a 10 per cent sugar solution fermenting at incu- 
bator temperature 
Period of 
Date of inoculation. fermen- Results of inoculation. 
tation. 
| Days. 
G5 Ve) O51 2) Ly RR Fa i ee Ee aan ee ene ee aaa 3 | European foulbrood produced. 
5 | No disease produced. 
Neptor OlO.2ot oso Sebel Bee Da ae eee 8 ee 7 
S72) 9 eC atl 42 5 1a eae eet care pee re ah Sey eee ae Se eee BIB 8 
7. SVL A 1 ee Oe oe ly ee a UR Me Soa RB 10 Do. 
GUNG SO: IOIG ao oe oie cee BS Bae ela aia RI hel ns peas ng 15 
Tahy bigs: 2 The Se eee a EL ci | 15 ; 
Aug. 24, 1915._..... Sas Dipai a Seow tee ise 2 Te Jee 24 | Do. 
TABIE X.—Bacillus pluton in a 10 per cent sugar solution fermenting at room 
temperature 
Period of 
Date ofinoculation. fermen- Results of inoculation. 
: tation 
Days. 
ane SU OIG. 2 Sap ee ap ee ein ee GR fee 9 | European foulbrood produced. 
FURY LC IO a aoe as ge nasa ah a aes aca ne ce 10 Do. 
Sepiss WOtp: ar as eee eee sae ca ee Se ec ees 10 Do. 
eb y Ziel GIGS e or eee ee ae ae Se a en ee | 14 Do. 
VEEN a Dis EON Ge eee sent a a a ee eae See 16 Do. 
Heeb ys Sik Ol GSS Sere es ee Se re ea ee | 17 Do. 
Sept Os 1OlG sine 22s sas eee ah a ee ee eee ae eee 11 | No disease produced. 
FUER DEG IGS eS Eo REESE SRG = ASAE aha a eRe | 14 Do. 
IA OS ON Giese Ai ere ae re or aan hie ae era | 21 Do. 
Anca IGl gree eas sh cere eye Je ASE St osc eS | 27 Do. 
Bs 3 Tee Be EY ape ee pel eae emp Raes  eaeee en Ae eee ee 32 Do. 
AEE 25 SIDES F595 Se a a saa Sea eee ee ee 4a Do. 
The experimental results contained in Tables TX and X show that 
Bacillus pluton is destroyed in a fermenting solution. At incubator 
temperature the virus was destroyed in from 3 to 5 days, and at room 
temperature it was killed in from 11 to 21 days. 
Similar experiments were made in which suspensions in 20 per 
cent honey solutions were allowed to ferment at outdoor temperature. 
The records obtained show that Bacillus pluton in this environment 
was still alive and virnlent after one month. 
RESISTANCE OF BACILLUS PLUTON TO PUTREFACTION 
Suspensions of the contents of stomachs from larve sick or dead 
of European foulbrood were made in a 1 per cent peptone solution. 
Soil was added to inoculate it further. Putrefactive changes were 
allowed to take place at incubator and room temperatures, respec- 
