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EUROPEAN FOULBROOD. 19 
TABLE V.—Resistance of Bacillus pluton to drying at refrigerator temperature 
Date of inoculation. Period of drying. Results of inoculation. 
Months. | Days. 
Oyo sale ia leh Way emigre Sapa aed ete ae ga ccplaee  Oteey SP ees ER 26 | European foulbrood produced. 
Septal lOnGe ence ee eae ne oie ea 3 28 Do. 
1h Keio hd ROW Gi 2a oes Uae AS a yl i Na NL ea Na a 6 12 Do. 
EUITLO RSE 1G Guers c Ah RU ERNE Ns NERS CO Ce 8 0 Do. 
hips PLO TO TICSD. eset Beatie ral vy A RRND ey IN AEN Ie 8 2 | Do. 
SUMO 2S ONO LS eee Lee Ae Me cays en ela iia ereiave ts 9 0 Do. 
AAU eh Sa GCS Sa NS ne ase UR hr ce a 10 7 Do. 
Sept eS LONG es sare isle eee eh NMA SURO Ee 10 18 Do. 
From Table IT it will be observed that Bacillus pluton in a dry 
film made from the contents of infected larvee resisted drying at 
incubator temperature for approximately one year. Table III shows 
that at room temperature, other conditions being similar, the re- 
sistance is approximately equal to that at incubator temperature. 
At outdoor temperature, as shown by Table IV, the resistance is 
again approximately the same. At refrigerator temperature, Table 
V, the experiments do not include the period at which Bacillus pluton 
is destroyed. In 10 months the organism was still viable and the 
results of the inoculations indicate from the character of the infec- 
tion produced after such a period that at refrigerator temperature 
Bacillus pluton will remain alive for a longer period than at the 
other temperatures studied. 
RESISTANCE OF BACILLUS PLUTON WHEN DRY TO DIRECT 
SUNLIGHT 
In experiments relative to the resistance of Bacillus pluton, when 
dry, to the direct rays of the sun, smears are made of the contents of 
stomachs of European foulbrood larve in Petri dishes or on slides, 
and after becoming dry are exposed to the direct rays of the sun. 
After intervals reckoned in hours inoculations are made by feeding, 
using the direct method. Infection resulting from such inoculations 
shows that the drying has not killed the organism. In Table VI 
experiments performed in this connection are summarized : 
TABLE VI.—Results of inoculation with Bacillus pluton in a dry film exposed to 
direct sunlight 
Period 
Date of inoculations. of Results of inoculations. 
exposure. 
Hours 
Teun ys QT Uae uae tatctcoe Cr NR ete oS RN peak ae hak ne meee te to 3 | European foulbrood produced. 
Sep Calis AG Tee ee aN ah EAN Re a Keo ae WER TER 6 Do. 
ARDY Rise OH ek se Se sir SUE i egeteh SI Se aN ad teres 7 Do. 
SOP) Ges2 2 MIG IN seein ey ieee men) hy Ute men ie Rae eS RRS 8 Do. 
Daly, QU LOMA ee enn MAGN Setar pale des f 10 Do. 
ESA) OM AUC MUD Se ie eis EES alia i seam ea glare eee SS Se 14 Do. 
NTRP STO ES ae es A eel NTs Po Se geo eae a ee RES 15 Do. 
TU VAQA OIA eee Sa acR ree tenes See eee Sa het et EN 16 Do. 
eX BNE 50 ICO) Wee ANAS, CoA Es GS eae eS i Oe EE leg 20 Do. 
Sep teWlOsNOl gas eae ouneee ee soe ee ce eg eee 20 Do. 
Sept Zool o us ieee aoe Ser cornet cicic Uc ours aan 21 Do. 
Ue ZOMG UA Sach alice aera ans cia yan tar siine (ay. SRC Sy AU 23 Do. 
Layo eee SHE OAL GE AeA ES SN DE NOUN tS | 27 Do. 
