18 BULLETIN 810, U. S. DEPARTMENT OF AGRICULTURE. 
fection results the germ is considered as having been destroyed. 
Observations have been made on the virus kept at incubator, room, 
outdoor, and refrigerator temperatures and shielded from the light 
in each instance. The experiments conducted with Bacillus pluton 
in these environments are summarized in Tables IT, III, IV, and 
V which follow: 
TABLE II.—Resistance of Bacillus pluton to drying at incubator temperature 
{ 
Date of inoculation. Period of exposure. Results of inoculation. 
1914 Months. | Days s 
UBL Sse Reon od ea a eee oS as ee ee eae 0 | European foulbrood produced 
SF UB yi MO seas Oe a eR eae eae ev mye pene pai ere a | em eee 17 Do. 
July: U7 ee EE SE Re Ee ee Oe eae 24 Do. 
DAT Vie LORS See ee ee eos ee ee ore Nit te 1 2 Do. 
PANT Se aes PIN aan ec ere en er Sau elt a ea ae 1 7 Do. 
Da UR ORE ho ie ee weer Bane yee Sona 1 17 Do. 
ESTE) OY Bes es tp a Ba eect Re Ste gar | Cee Reh ae eee 2 0 Do. 
Sep isdlOs eas er Ses eae er eee ees | Z 11 Do. 
POLS) OU EaR A ec enter eT ec SO hay pI ed oct 3 0 Do. 
1915. 
Hy Abb ee) A) ea agen ys, Ae Mis Sepa sa cle Sane mena eens ei 9 10 Do. 
SST yp Se a ply he et ace 12 2 Do. 
FST EE aay Se phe ster EC he tele an Sean Oe RDG ge 12 0 | No disease produced. 
SAN OL aie ears ea et ae Sin Ie SE Ee eg Nad 14 18 Do. 
Sep Gc ae ss ew ek Pe oe cm De ee Se 14 20 Do. 
1916 
DO Pt Seek es eae ee nae REE ty oes 24 | 0 Do. 
| | 
TABLE III.— Resistance of Bacillus pluton to drying at room temperature 
Date of inoculation. Period of drying. Results of inoculation. 
l 
Months. | Days. 
ysl OPA Sees Ree eee See ee ee 1 | 1 | European foulbrood produced. 
PSL) On Fase Ka} 0 Mb) [ce ere ee es oh er eee a | 1 | 21 Do. 
Sepecde LOLA rte re eee ae re eee 2 8 Do. 
SO Pts 28 ol GUA ayes oe ese ea a eles pera 2 21 Do. 
SLE) OL Farad el AE) Ke Soin heey reo nN By Oe ee Rogey nas 3 | 0 Do. 
OCT SGU U4 ee elie aie cep Ie ne eee es Pe ae ok Op 3 | 14 Do. 
Jars 20 M1015 Ss eles FN A ee 9° 10 Do. 
TealiyeO e115 see ee esa eee ns Ss et 12 | 6 Do. 
PANU ES SAO VA ie ere ae ates i Ee a | 11 | 13 | No disease produced. 
ARG HAYS 7A ROD GOS PR Sa Es See pes Se tat a Se Pema ne, ee 11 | 15 Do. 
JUNE 2a e1 915 ose e ke a Soe eee een eo Se ee 11 | 18 Do. 
SCO AOI G he a ae eo Ee ee ey epee 12 | 2 Do. 
Septal 7N1Ol pace eae Saas ite ane cae ee 14 | 10 Do. 
DEP lse20 O15 Sei aie eee wey ese 14 18 Do. 
DOPE SLOG kee Steen ee ee Oe ee eee yom ene ee 24 0 Do 
Oe SE i fa Ra Se cad bt ak cy ae 36 0 Do 
TABLE 1V.—Resistance of Bacillus pluton to drying at outdoor temperature 
Date of inoculation. Period of drying. Results of inoculation. 
Months. | Days. | 
So 47 I) ee eee on Seared ne ce soe SaaaadaDaSosisoos saa00C 33 | European foulbrood produced. 
Sentclo; (Oita rey enc sone tener oe Ui eteal| eee mereeree 47 Do. 
Oct 18; WOR el a 8 ede es dons res ie La eee S| a 74 Do. 
MA Vi2Gs MOUS ure oS ine cain ta cig eee ana ears are ales 9 21 Do. 
PUM OA Oe LOTS ese ee Re ea ee 10 18 Do. 
May 17, 1915.._--- ERG SE (aa te UE ese ON 13 17 Do. 
Aug SS ed O15 228s Soe e koe ee ee ee ORG a aaa 12 3 | No disease produced. 
SA brig) Lr /oen Ue i ach ees Se caet eR. Bane eo es Ee 12 Lg Do. 
Jane 23s Ol Getty See ea ene cin nee tater eee PS ye eee eam oe Do. 
