EUROPEAN FOULBROOD. | 13 
Glucose gelatin plate.—At refrigerator temperature and within 3 days, the 
surface colonies begin to liquefy the gelatin, each liquefied area appearing 
somewhat as a minute drop of water. 
Agar slant.—In one day numerous gray colonies cover the inoculated surface. 
“Bouillon.—Within a day the medium is uniformly and moderately clouded. 
Fermentation.—In glucose, lactose, saccharose, levulose, maltose, and man- 
nite bouillons, a uniform clouding of the media occurs. The growth takes place 
in both arms of the tube, but is heavier in the open one, Considerable acidity, 
but no gas, is produced. 
Milk.—Milk is rapidly coagulated. Digestion of the coagulum follows. In 
from 3 to 5 days more than one-half has been changed. Within 24 hourg the 
color is discharged in litmus milk, except at the top of the medium. In other 
respects it is like the plain milk. 
Potato.—No visible growth. That growth in the potato water takes place is 
confirmed by microscopic examination. 
Gelatin stab.—Liquefaction along the line of puncture is appreciable after one 
day. In four days a cylinder of liquefied gelatin 1 cm. in diameter surrounds 
the original line of puncture and soon extends to the walls of the tube. 
Pathogenesis.—No disease results when 
the brood of bees is fed cultures of 
Streptococcus apis either by the direct 
or indirect method. A rabbit and two 
guinea vigs inoculated with a pure cul- 
ture of Streptococcus apis were not sus- 
ceptible to infection with the species. 
BACTERIUM EURYDICE 
The presence of this species in 
European foulbrood was pointed 
out by the writer in an earlier pub- 
lication (15). Among the second- 
ary invaders in larve infected with ~ 
Bacillus pluton, Bacterium eury- BGs ea otc nr ey ate: 
dice is one of the earliest to be found. It is often present in consid- 
erable numbers. In plating for the species the stomach contents from 
larvee sick, but not dead, of the disease should be used. In studying 
this species cultures were isolated which in some respects differed 
from it. Whether these are different species or belong to a group of 
which Bacterium eurydice is a representative has not been definitely 
determined. 
To isolate Bactertwm eurydice the plating has been done with glu- 
cose agar. Incubation must be carried out at room temperature. 
Growth of the species is always slow and never luxuriant. Under 
favorable conditions colonies are visible after one day. To preserve 
cultures they must be renewed frequently. 
Occurrence.—Bacterium eurydice is frequently present in larve sick or 
recently dead of European foulbrood. 
Glucose agar plate—To the naked eye the surface colonies are slightly 
convex, smooth, and glistening. They are from 1 to 2 mm. in diameter, cir- 
