REPELLENTS FOE PROTECTING ANIMALS FROM FLIES. 19 



greatly reduced. There was a very slight odor of citronella at that 

 time. 



July 10, 1913, applied the mixture to a cow by means of a brush. 

 Used about 6 ounces of oil. July 11, about 22 hours after applica- 

 tion, oil was present on the neck and along the back. There was 

 no odor of citronella. There was little or no protection as indicated 

 by the presence of many hornflies on the underside of the abdomen, 

 and the presence of many stable flies on the legs. 



It is noted from the above tests that the mixture used is a powerful 

 repellent, but that its effect does not last more than a few hours. 



TEN PER CENT OIL OF SASSAFRAS IN COTTONSEED OIL. 



June 19, 1913, a mixture of 10 per cent oil of sassafras in cottonseed 

 oil was applied to a calf by means of an atomizer. There was a pro- 

 nounced repellent action which, however, had disappeared at the 

 end of a few hours. 



July 2, 1913, the same calf was again treated. An hour or so later 

 a repellent action was still present. The calf was troubled very 

 little with flies as compared with the other animals. July 3, there 

 was no odor or protective action noticeable. 



July 3, 1913, treated a cow with the mixture. Used about 3 ounces. 

 The repellent action was marked. An hour or so later the repellent 

 action was greatly reduced and there was no odor of sassafras. 



July 10, 1913, applied the mixture with a brush to the above cow. 

 Used about 5 J ounces. July 11, about 22 hours later, a little oil was 

 present on the neck, withers, and just behind the withers. Many 

 hornflies were present on the front legs and on the underside of the 

 abdomen. 



The above tests show that the mixture has a marked repellent ac- 

 tion, but that this only lasts for a few hours. 



TEN PER CENT OIL OF CAMPHOR IN COTTONSEED OIL. 



June 19, 1913, a mixture of 10 per cent oil of camphor in cotton- 

 seed oil Avas applied to a calf by means of an atomizer. A few hours 

 afterward some protective action was still noticeable. 



July 2, 1913, the same calf was again treated. An hour or so later 

 the calf was still protected from flies. 



July 3, 1913, no protection was noticeable in the case of the above 

 calf. A cow was sprayed with the mixture. Used 2^ ounces. There 

 was a marked protective action. An hour or so later the protective 

 action was greatly reduced. There was no odor of camphor. 



July 10, 1913, applied the mixture to the above cow with a brush. 

 Used 5 ounces. July 11, about 22 hours after application, a little 

 oil was present on the neck and along the back. There was no odor 



