8 BULLETIN 245, U. 8S. DEPARTMENT OF AGRICULTURE. 
The results given in Table IT show a 97 and 98 per cent larvicidal 
action with the two stronger solutions, and in the case of the 1 to 200 
dilution two cages showed a 99 per cent and one an 80 per cent 
larvicidal action. The 1 to 50 and 1 to 100 strengths showed an 
increased number of bacteria. The amount of water-soluble nitrogen 
and ammonia is noticeably increased in the manure treated with the 
strongest solution of aniline, with no apparent action on the bacteria. 
The increases of the water-soluble nitrogen and ammonia are proba- 
bly due to nitrogen in the aniline added. Aniline, among other sub- 
stances, was tried in some cage experiments at New Orleans, the 
results of which are given in Table III. 
TaBLeE III.—Destruction of fly larve in horse manure. Results with aniline, hellebore, 
and nitrobenzene; cage experiments at New Orleans, La., 1914. 
| 
‘ 
Series. | Treatment of 8 bushels of manure, using 10 gallons of liquid. oe d ee 
merged. ns. 
Number. Number. 
A | Aniline, 1-200... .- A oi Ee se SEER SACO RUS ORE ae Gp SGeTE 3, 738 2,380 
1 Bal pAmiline 41-400... soe e So sae ae oe te a Se eee ee ee eee 6, 030 12, 853 
Cae GOS A eae erage gees See Rees NUE Cri St Seda re Sui oe aaa 5,070 9, 856 
A Hen erore, sTound, pound to 10'gallonsiwater..-----2-=-----=-------= 1,122 4, 034 
10g)B |-----dO--..---------- +--+ - 2-22 = eno tee ee eee ” 820 4, 567 
C iiellenore, ground, + pound to 10 gallons water... ......---.--.-------- 1, 130 1, 285 
IDs Ee O eae Sek Bea See nnn eee We a eee eee eee ” 974 1,930 
fA Noes. 1 pound, and 4 pound fish-oil soap to 10 gallons water. 3,390 | 2, 989 
100)8 ac DS BS SR Ge SN eee hn Uy ear rr 1, 692 3) 647 
C | Nitrobenzene, 4 pound, ana + pound fish-oilsoap to 10 gallons water... 1,319 2, 042 
11053 Control (wateronly) ao. oe as od oat eae SN aos See eee eas 1,085 560 
3a | Sake (N Ga aaa Sees nae pee a ee ne eee ete ea nee 5, 888 4,835 
(AS Aci ihn 62st 200) Seeger ee oe Sore ere eee ae Sa Ae ee nee ree 5, 076 4, 462 
1114,B Aniline, A OO Bee Sane CO ee we Seine eee eee ere eee eee 12, 667 8, 296 
(Oh Se Bee GORE. - See er abl hh eee ae oe + se nba Ue Sane eee ee cee Oe 12,309 19,675 
A Beare, eround, = pound-to 10 callonsiwater... Sssesa=-- nee ee 11, 803 10, 794 
119) B |-----d0....--------------- 2-2-2 een 17,197 8,179 
Cc iieilebore, ground, + pound to 10 gallons water... ..........-.......--- 20, 067 9,315 
TOYS 28 S20 (NS ee es ee On SN eR ee anne 3c SG aH ees Sootae 17, 838 268 
: N itobenzene, 1 pound, and 4 pound fish-oil soap to 10 gallons water. 2,395 10, 132 
TITS TB 35a [ees LO eee es aarti eh <a en DRS RS Se eae ot Saar ae ence 12 211 
N concn 34 pound, and + pound fish-oilsoap to 10 gallons water..-- 9,678 11, 165 
114 fA Control (water SOMLY, pene se ens Le ee ee re ae 19,366 | 11,419 
UBalo ce CORE Seo ctetin narra oe eae een Saisie Sasi sateen Seca te se 18, 838 | 10, 726 
The cages were the ones used the previous year and, as the figures 
show, large numbers of larve escaped. Apparently these results 
show a low larvicidal effect of aniline, but since the condition of the 
cages was unsatisfactory they should not be compared with the 
others, especially as they are the only ones, either in cages or open 
piles, where poor results with aniline were obtained. 
Open-pile experiments at New Orleans, using a 1 to 200 dilution of 
aniline, killed 85 and 99 per cent of the fly maggots (Table IV, series 
46, A and B); 1 to 400 dilutions in two piles gave 79 and 83 per cent 
larvicidal effect (Table V, series 41, C and D); and two piles treated 
with aniline, 1 to 500, ave an 84 per cent destruction of larve 
(Table V, series 49, A Baal B). 
