LOSS OF NICOTINE FROM NICOTINE DUSTS | B 
The dust mixtures were made up as follows: A quantity of the 
nicotine solution sufficient to produce a dust of the highest nicotine 
content employed in the tests was added to the carrier. After putting 
this mixture through a suitable mixing and sifting machine, a portion 
was reserved for the highest strength, and two other portions were di- 
luted with the carrier and again put through the sifter and mixer. 
The mixtures were then analyzed and packed in the following types of 
containers: Pasteboard boxes made of two thicknesses of cardboard, 
with a slip cover of the same material; heavy cylindrical paraffined 
boxes, having close-fitting covers of the same material, a type much 
used commercially for semiliquid materials; glass jars with screw 
top and rubber ring; and small canvas bags. They were stored at 
room temperature. 
The samples were analyzed at definite intervals covering a period 
of a year or longer. In the earlier part of the work all Saeieers were 
made by the official silicotungstic-acid method (Official and Tenta- 
tive Methods of Analysis, Association of Official Agricultural 
Chemists (1920), p- 65). As the number of samples increased and 
the time involved in the steam distillations became great, experiments 
were made to determine whether or not the nicotine could be washed 
out of the dusts and precipitated directly without undue loss in 
accuracy. It was found that by direct extraction with water prac- 
tically the same results were obtained as by steam distillation. This 
procedure therefore was adopted for the later work. 
EXPERIMENTAL RESULTS 
The results of the analyses of the nicotine dusts are given in 
Tables 1 to 10, inclusive. 
Three strengths ‘of nicotine sulphate-kieseleuhr dust (Table 1) 
were used in the first series reported. 
TABLE 1.—WNicotine in nicotine sulphate-kieselguhr dust 
Strength 1 Strength 2 Strength 3 
ae Hs P Paraf. C Paste-| Paraf Cc Paste-| Paraf- C 
analysis aste-| Paraf- an- aste-| Paraf- an- aste-| Paraf- an- 
board | fined ee vas || board | fined See vas || board] fined a vas 
box | box | ! bag || box | box | J bag || box | box | J bag 
1922 Per ct.| Per ct. | Per ct.| Per ct.|| Per ct. | Per ct.| Per ct. | Per ct. || Per ct.| Per ct.| Per ct.| Per ct. 
Mar iS 442. 4aNG Ce 7.1 CAT 5. 12 5. 12 5-12 Oo D2 2. 24 2. 24 2. 24 
YS ees ce To 29 E56! | len® |a= oes = 5. 26 5. 23 5-09-|-2 see 2. 25 2. 28 2. 26 220 
Is 8 a a Fy eC Pilea as nl a fa Us 7.14 5. 20 5.28 | 5.14 5. 05 2. 28 Dy Ppcer shales eae 
WURO psa | OD 1640) |.9 4. 09) 7.32 520) || 5.39) Satie. 30 2.30} 2.31] 2.24 2. 28 
SOR b oe see eee ee so |[Seeiwes 5. O01 5. 01 5. 14 2.25 2.-38-|-- 2: 25 2. 34 
INO Vs S22 o-). tee G84 122 6.80 | 6.64 4.84 | 4.86] 4.76] 4.98 2.08 | 2.10] 2.14 2. 23 
1923 
INOW. S2eeeoees GO dee ee 6.18 | 5.78 3.89 | 4.03] 4.46] 3.89 1.85} 1.92] 1.90 1. 74 
The results in Table 1 show that the rate of liberation of nicotine 
from kieseleuhr is slow. No loss had occurred at the end of three 
months.2 Even in the canvas bag, the change in nicotine content 
2 The higher strengths show, in some cases, an increase in the nicotine content after three months, which 
may be accounted for, partially at least, by the loss of moisture from the samples and consequent relative 
concentration of nicotine in the samples. 
