| i : 
: roe: 1886, Muroscopy. 409 
the anterior end into a drop of water ona slide. If this is done 
in early spring, all the phases of fissiparism may be obtained, to- 
gether with encysted stages. The best time, however, for study- 
ing the process of fission is during the winter. If a frog is kept 
in a warm room twenty-four hours and ‘fed well, it will be found 
that fission of the Opalinz, if any are present, has advanced to the 
j point T encysting, and a complete series of stages may be readily 
i obtaine 
| 
Í 
i 
Material for the study of the development is obtained from 
young tadpoles (5"™ and upwards in length) collected from their 
natural haunts, or better, raised from the egg and fed with Opalina 
aqueus should be inclosed air-tight. 
B) For the study of the caryokinetic multiplication of nuclei, 
T which is the leading feature in the development of multi-nucleate 
: orms, preparations may be made in the following manner: 
‘a *Tess out a portion of the fæces from the anterior end of 
4 e rectum on to a slide, adding a drop of water. 
enS ond its edges, and the space as thin as possible in 
3) By means of a brush make a border of picric acid (satu- 
3 thig cover, while it is drawn away with blotting paper from the i 
Aie he nag Great care should be taken not to add the water — 
would Ais ey it is drawn away, as the raising of the cover ; 
| tlhe to en poral hours, and must be closely watched from begin: : 
dia &) Stain with Grenacher’s alum. carmine or hematoxylin, — 
a ng a border of the dye around the cover, as was done with ~ 
ane 
