cernible as such, but instead, opposite the centre of the 

 concavity of the parasite, was grouped a number of 

 purplish granules which at times extended beyond tliis 

 area as scattered grains. A vacuole was often associated 

 with the mass. In the heavily parasitised frog, unusual 

 appearances were noticed which suggested either that 

 these granules were not of the same nature as the nucleus 

 proper, (our specimens were all Giemsa stained), or else 

 that portion of the nuclear chromatin separated itself from 

 the nucleus proper. The appearances were as follows :— 

 The group of granules referred to seemed to emigrate into 

 the bulge lying first of all close to the parasite proper, 

 sometimes in a non-staining vacuolar-like space, and free 

 in the bulge. In many of these cases, the true band-like 

 nucleus itself could be distinctly recognised quite apart 

 from the mass, and situated more towards one end of the 

 parasite, the bulge being then a little to the other end. 

 Further, in the same specimen, a number of rounded, com- 

 paratively large, purplish chromatic-like bodies were seen 

 (one in each cell — once two in a cell) free in the protoplasm 

 of the red cell, occasionally close to the bulge, but more 

 often far removed from this and even to be seen opposite 

 the convex border of the parasite. It was noted that in these 

 cases, the bulge was slightly developed or absent. The 

 protoplasm of the parasite proper seemed often sharply 

 differentiated from this bulged area, and in some cases the 

 outline of the body proper could be traced tras versing the 

 bulge, distinctly following the regular curve of this side. 

 There were, further, sometimes differences in staining 

 reactions by Giemsa's method: the parasite itself staining 

 a definite bluish tint, with or without granules, and the 

 bulge being a paler blue or colourless and vacuolar like, or 

 with a colourless centre, but a deeper blue periphery. In 

 some free forms of the parasite which were seen, it is note- 



