worthy that the bulge was not noticeable. In the heavily 

 infected frog, some mitosing red cells were met with. 



It is difficult to know what the bulge and chromatic-like 

 mass represent. It must be borne in mind that the speci- 

 mens obtained were dry blood-films stained by Giemsa's 

 method and that, at the time, we were unable to adopt the 

 more exact methods for fixation or staining advocated by 

 Minehin. The question of distortion from drying and the 

 accentuation of size of chromatic structures by the stain 

 must be taken into account. Allowing for these imperfec- 

 tions, however, it is obvious that the bulge exists, and that 

 masses are often present in the parasite which resemble 

 chromatin by Giemsa staining, and yet are sometimes dis- 

 tinct from the nucleus. We have seen these chromatin- 

 like masses collecting opposite the bulge, passing into it, 

 and finally grouping in it, and we have further seen free 

 bodios sug-o-Uti.u- that these masses Anally escape from the 

 bulge and wander into the host's protoplasm. Is the 

 chromatin-like mass excreted through the bulged area? Is 

 it really chromatic in nature and a device for the reduction 

 in the amount of chromatin of the nucleus proper ? These 

 are points which we hope in the future to be able to resolve. 

 It should be noted that these remarkable appearances 

 associated with the bulging were noted in only one of the 

 infected frogs, and in this specimen the infection of red 

 cells by the parasites was very heavy. 



The animal occupied various positions in the host cell, 

 the more common situation being diagonal. Occasionally 

 it lay across the host cell between the nucleus, which 

 might or might not be displaced. Sometimes its position 

 was between the nucleus and the side of the cell. In one 

 instance it was located longitudinally between the nucleus 

 and one end of the erythrocyte. Not infrequently the host 

 nucleus was more or less displaced, but in no case was it 



