36 BULLETIN 817; U. S. DEPT. OF AGRICULTURE. 
October 31, 1917: Two days after feeding, second guinea pig etherized about 
2 p.m. and when entirely anesthetized the abdomen was opened and blood drawn 
by a syringe directly from the portal vein, the blood being delivered into a 
_test tube containing 5 cubic centimeters of a 5 per cent solution of acetic acid 
and centrifuged. The second specimen of the sediment taken for examination 
from the bottom of the tube contained two larve about 0.28 mm. long. 
It appears from the experiment above that 48 hours after feeding cultures of 
Ascaris eggs, Ascaris larve may be found in the blood in sufficient numbers to 
be demonstrated in the portal circulation, but the results of a single experiment 
such as this ean not be accepted as conclusive. 
Experiment No. 12. 
November 3, 1917: Fed 3 guinea pigs with culture of Ascaris suum. 
November 5, 1917: Two days after feeding killed and examined blood of first 
guinea pig, keeping portal system and systemic system separate, using 3 per 
eent acetic acid, same technique as in previous experiment. Both liver and 
blood negative. 
November 6, 1917: Three days after feeding killed and examined second 
guinea pig, drawing bleod from pulmonary artery. One larva seen. Two 
larve in lungs; none in liver. 
November 9, 1917: Third guinea pig died from pneumonia six days after feed- 
ing. Many larve in lungs, several in trachea, one in esophagus, one in small in- 
testine, none in liver. 
It appears from the above experiment that the larve after leaving the liver 
are carried by the systemic circulation to the heart and thence by the 
pulmonary artery to the iungs. In this experiment as early as three days after 
infection a few larve had already entered the lungs and as none were found 
in the liver it is possible that most of the-larvz were in the blood stream on 
the way to the lungs. The results of a single experiment such as this, how- 
ever, can not be accepted as conclusive. 
Experiment No. 13. 
November 22, 1917: Three guinea pigs injected subcutaneously with Ascaris 
SUUM eggs. : . 
November 30, 1917: Killed first guinea pig eight days after injection. Larva 
in lungs, 0.5 mm. long. Abscess at site of injection contains Ascaris eggs, the 
embryos dead and unhatched. Spleen, trachea, and esophagus negative; lungs 
with hemorrhagic spots. 
December 4, 1917: Killed second guinea pig 11 days after injection. Larva 
in lungs, 1.5 mm. long; one anterior lobe of lung chocolate brown; a few 
hemorrhagic spots over the rest of the lungs. Spleen, trachea, and esophagus 
negative. Unhatched eggs containing vermiform embryos in abscess at site 
of injection. 
December 4, 1917: Killed third guinea pig 11 days after injection. Lungs 
showing extensive inflammation and covered with dark hemorrhagic spots. 
Larvee in lungs; one measured 1.3 mm. in length. Larvee in trachea measuring 
1.5 mm. Spleen and esophagus negative. Eggs in abscess under skin at site 
of injection. Some empty shells. Embryos in eggs dead. 
The experiment of injecting Ascaris eggs beneath the skin was repeated, 
six guinea pigs being used. Similar results were. obtained, the larve being 
found in each case in which the lungs were examined a week to 10 days after 
injection of the eggs. 
