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LIFE HISTORY OF ASCARIS LUMBRICOIDES. : 33 
December 19, 1917: Killed the last mouse 296 days after feeding. A small, 
encapsulated tumor, superficial, in liver near the margin contained degener- 
ated larve and a waxy or caseous substance. No definite calcification. 
Experiment No. 5. 
June 28, 1917: Fed 12 white mice at 11 a. m. eggs of Ascaris suum on 
bread, after starving 24 hours. 
June 25, 1917: Killed the first mouse 51 hours after feeding. In stomach 
2 unhatched eggs; in small intestine a few unhatched eggs. Lungs and spleen 
negative. Liver heavily infested with larve 0.28 to 0.33 mm. long. In large 
intestine unhatched eggs containing viable embryos. 
It is evident that within 51 hours large numbers of the larve have lodged 
in the liver, but apparently the lungs have not yet been invaded. It is in- 
teresting to note that a number of eggs still remain in various parts of 
the digestive tract and, apparently, are in the process of passing out, since 
they are found fairly numerous in the large intestine. 
June 28, 1917: Killed second mouse 5 days after feeding. Liver heavily 
infested. Larve from the liver were kept alive in salt solution for 24 hours, 
but all died within 48 hours. Lungs, spleen, small and large intestines negative. 
Larve in liver measured 0.28 to 0.34 mm. in length. 
June 29, 1917: Third mouse died of pneumonia 6 days after feeding. In- 
tense congestion and hemorrhage by diapedesis. No larvee in the liver, spleen, 
or intestines, but very numerous in the lungs. Larve in lungs measured 0.34 
to 0.4 mm. in length. 
June 30, 1917: Fourth and fifth mice died of pneumonia 7 days after 
feeding. Lungs heavily loaded with larve, 0.29 to 0.66 mm. in length. Other 
organs not examined. 
July 2, 1917: Sixth mouse died from pneumonia 9 days after feeding. Lungs 
heavily infested with larve, 0.56 to 0.86 mm. in length. Liver heavily in- 
fested with larve, 0.56 to 0.86 mm. in length. Small intestine, 3 larve. HEsoph- 
agus, numerous larve, 0.9 to 1.45 mm. in length. Spleen, 1 larva, 0.86 mm. long. 
Cecum, 4 larve, 0.88 to 0.46 mm. long. 
July 3, 1917: Seventh mouse died 10 days after feeding. Lungs heavily in- 
fested ; larvz 0.6 to 1.12 mm, in length. Small intestine, numerous larve, 0.83 
to 1 mm. in length. Large intestine, several larve, 0.75 to 1.45 mm. in length. 
Spleen negative. 
Most of the larvee remained actively motile in the tissues for 24 hours after 
the animal died, but all larve were apparently dead 48 hours after the death © 
of the host. 
July 5, 1917: Killed eighth mouse 12 days after feeding. Lungs heavily 
infested. 
Of the 4 mice remaining 3 were Jost track of and 1 was still alive June 5, 1918. 
Experiment No. 6. 
September 22, 1917: Fed 6 white mice with eggs of Ascaris suum on bread. 
September 24, 1917: One mouse died 2 days after feeding. No larve in 
liver. 
September 29, 1917: Second mouse died 7 days after feeding. Not examined. 
October 4, 1917: Third mouse died from pneumonia 12 days after feeding. 
Numerous larvze in lungs. Other organs not examined. Two mice born to one 
of the mice fed September 22. 
