a2 BULLETIN 817, iW. 28. DEPT. OF AGRICULTURE. 
Experiment Ne Zs 
February 28, 1917: Fed 5 mice with eggs of Ascaris swum on bread. 
March 3, 1917: Second feeding. | 
March 10, 1917: One mouse died with symptoms of pneumonia; no post- 
mortem. ; 
March 14, 1917: Two more mice died from pneumonia, 14 days after the 
first feeding, 11 days after the second. The post-mortem showed a heavy in- 
vasion of the lungs in both mice. In one of the mice no larvze were found in 
the liver or small intestine, 5 larvee in the cecum; in the other, 2 larve in the 
liver, 36-in the small intestine, 4 in the cecum. 3 
The larve in the lungs were of two rather distinct sizes evidently correspond- 
ing to the different dates of infection. The larger size varied from 0.53 to 0.88 
mm., the Smaller from 0.28 to 0.33 mm. in length. 
<iaik ch 23, 1917: Examined feces of remaining mice for larve. Newtsa 
“March 26, 1917: Killed_ fourth mouse 26 days after first feeding, 23 days 
after second feeding. Post-mortem: Lungs, 15 larve 1.3 to 14 mm.; ape 
2 Jarvze: 0.8 mm.; thyroid, 1 larva 0.7 mm.; liver, 2 larve 0.8, 0.9 mm.; small 
intestine, 1 have 1.2 mm.; cecum, 7 larve 1. 6-1.9 mm, 
March 28, 1917: Examined feces of fifth mouse. Negative. 
““Xpril 21, 1917: Killed fifth mouse. Negative. 
Experiment No. 3. 
April 27, 1917: Fed 6 mice at 11 a. m. Ascaris suum eggs on bread. 
April 28, 1917: Killed 2 mice 28 hours after first placing food before them. 
One negative; in small intestine of the other 1 Ascaris egg with shell intact, 
in stomach 4 Ascaris eggs containing living embryos. 
May 2, 1917: Killed third mouse 5 days after feeding. Several larvee in 
liver, 0.4 to 0.55 mm. long; length of esophagus 0.1 to 0.8 mm., distance from 
anus to tip of tail 0.07 mm., greatest width 0.02 mm. Spleen, lungs, heart, 
kidney, stomach, small intestine, cecum, negative. 
May 5, 1917: Killed fourth mouse 8 days after feeding. Larve in liver only, 
0.4 mm. long. 
May 10, 1917: Killed fifth mouse 18 days after feeding. Larve in liver, 
lungs, under peritoneum in various parts of abdominal cavity, in feces. Larve 
in feces were 1.2 to 1.75 mm. long. Length of esophagus 0.18 to 0.23 mm. 
Anus to tip of tail 0.1 mm. Larve in lungs 1.7 mm. long. Length of esopha- 
gus 0.24 mm. Anus to tip of tail 0.08 mm. Nerve ring 0.11 mm., excretory 
pore 0.14 mm. from anterior end of body. Maximum width of body 0.06 mm. 
February 12, 1918: Killed sixth mouse 94 months after feeding. Negative 
except for a few small black spots on the lungs, like the remains of old hem- 
orrhages. 
Experiment No. 4. 
February 26, 1917: Fed 8 mice Ascaris suum eggs on bread. 
March 2, 1917: First mouse died 4 days after feeding. Negative. 
March 17, 1917: Killed second mouse 19 days after feeding. Seven larve 
in liver, 8 in spleen, none in other organs. 
March 26, 1917: Killed third mouse 28 days after feeding. Negative. 
April 19, 1917: Killed fourth mouse 52 days after feeding. Negative. 
July 5, 1917: Killed fifth mouse 129 days after feeding. Negative. . 
November 10, 1917: Killed sixth mouse 257 days after feeding. Negative. 
December 3, 1917:.Killed seventh mouse 280 days after feeding. Negative. 
