14 SOME FUNGOUS DISEASES OF ECONOMIC IMPORTANCE. 



stages of the supposed disease were not secured, but in all the fruits 

 examined fungous mycelium was found and this mycelium uniformly 

 developed on the surface of the fruits as a blackish Stemphylium. 



CULTURES OF THE FUNGUS. 



The fruits after having been placed in 1 part of mercuric chlorid, 

 by weight, to 500 parts of water for fifteen minutes and then thor- 

 oughly washed in distilled water were either placed entire in steril- 

 ized glassware, or sections of the fruits were removed by flamed 

 scalpel and forceps and dropped in culture flasks. From these the 

 same Stemphylium has always developed, even from sections of inner, 

 apparently healthy tissue. Inoculations from pure cultures always 

 developed satisfactorily on healthy experimental fruits when kept 

 under favorable conditions of moisture and temperature, but it is not 

 thereby claimed that the species to be described is of economic 

 importance or a necessary factor in the so-called " end-rot," although 

 it was always observed associated with this trouble. 



DESCRIPTION OF THE FUNGUS STEMPHYLIUM CITRI. 



Vegetative mycelium long, hyaline, later becoming dark, 4 n in diameter, sep- 

 tate ; conidiophores short ; conidia dark brown, subglobose or oblong, apiculate, 

 irregularly muriform, 20 to 30 fi by 12 to 15 n, usually in chains of three with 

 isthmus cells short, hyaline. 

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