PRINCIPAL PHYSIOLOGICAL TESTS. 27 



Ott and Harris a had tried this antagonistic action of these two 

 bodies, but did not push the matter as far as Cameron. A similar 

 antagonism with cholin had been pointed out by Lohmann. b After 

 having determined the amount of active principle on the dog, rabbit, 

 or cat by the simple blood pressure method, as a check to this method 

 it may be advisable to inject the amount of the unknown solution cor- 

 responding to 0.0075 mg. adrenalin mixed with a solution of 0.6 mg. 

 nitroglycerin into the jugular vein of a rabbit. I do not consider 

 this additional test necessary, but the results of Cameron are cer- 

 tainly worth controlling. 



Gurber c pointed out the presence of a depressor body in the supra- 

 renal extracts. A portion of this action may be due to cholin and a 

 portion to unknown bodies. d Extracts containing these depressor 

 bodies are especially toxic. In crude extracts the depressor action 

 of these bodies, and also of certain inorganic salts, should be kept in 

 mind. 



At the end of the experiment the animal should be killed by inject- 

 ing chloroform into the veins, so that the most humane person could 

 find no objection on the score of cruelty. 



In the case of a mixture of adrenalin and peptone the action of one 

 follows that of the other, and it is therefore unsuited for antagonistic 

 study/ 



a Ott, I., and Harris, S. B. Physiological action of adrenalin chloride. Therap. Gaz., 



vol. 27, p. 378, 1903. 

 b Lohmann, A. Cholin, die den Blutdruck erniedrigende Substanz der Nebenniere. 



Arch. f. Gesam. Physiol., vol. 108, p. 222, 1907. 

 cMetzger, L. ZurKenntniss d. wirksam. Substanzen d. Nebenniere. Dissert., Wtirz- 



bnrg, 1897, p. 17. 

 d Hunt, R. Further observations on the blood pressure lowering bodies in extracts 



of the suprarenal glands. Proc. Amer. Physiol. Soc, Amer. Jour. Physiol., 



vol. 5, p. vi, 1901. 

 Marino-Zuco, F., and Dutto, U. Chem. Untersuch. ii. die addison'sche Krank- 



heit. Untersuch. z. Naturlehre. Herausg. von J. Moleschott, vol. 14, p. 617, 



1892. 

 Lohmann, A. Cholin, die den Blutdruck erniedrigende Substanz der Nebenniere. 



Arch. f. Gesam. Physiol., vol. 108, p. 215, 1907. 

 Pari, G. A. Azione locale dell' adrenalina sulle pareti dei vasi, ed azione delle 



minime dose di adrenalina sulla pressione del sangue. Arch, di Farmacol. 



Speriment., vol. 4, p. 175, 1905. 

 Vincent, S. Nature of the physiologically active substances in extracts of nervous 



tissues and blood, with some remarks on the methods of testing for cholin. 



Jour. Physiol., vol. 30, p. 143, 1904. 

 « Hamburger, W. W. Action of intravenous injections of glandular extracts and 



other substances upon the blood pressure. Amer. Jour. Physiol., vol. 11, 



p. 282, 1904. 



