24 SUPRABEUAL GLANDS. 



The persistency of the rise gives a better idea as to the intensity of 

 the action: thus, using a drum which traveled at a rate of 0.28 cm. 

 per second, after injecting 1 c. c. of the solution specified the drum 

 traveled 30mm. before the blood pressure returned to its original level — 



Alter 2 c. c. it traveled 40 mm. 



After 3 c. c. it traveled 102 mm.. 



After 4 c. c. it traveled 130 mm. 



After 5 e. c. it traveled 150 mm. 



Young dogs are claimed to be more responsive to adrenalin than 

 older dogs having the same weight.' 7 



The quantitative work which has been carried on with adrenalin 

 has been performed with the adrenalin of commerce. This was shown 

 to contain extraneous matter (phosphates : . as one would expect 

 from the method of isolation described by Takamine. so that it does 

 not seem rational to standardize against a body which is not chem- 

 ically pure and which the manufacturers themselves do not label as 

 c. p. The standard used should be the highest grade of active prin- 

 ciple known. Abel's latest method, as acknowledged by Pauly , or 

 adrenalin as prepared "by Aldrich's method seems to answer this 

 demand. This adrenalin (Aldrich's) can perhaps be obtained from 

 the manufacturers, but at a higher price. A supply of this high 

 grade adrenalin (epinephrin should be kept on hand in a vacuum 

 desiccator and preserved in the dark. When the test is to be made, 

 a few milligrams of this are accurately weighed off and dissolved 



in water with a little over the calculated amount of HCIjq, con- 

 stantly shaking.' and made up in a proportion of 1 to 50.000, the 

 solution placed in a Florence flask and gently heated on the bath 

 to body temperature (37 ^~ C. . One-half of a cubic centimeter of 

 this solution would correspond to 0.001 mg. per kilo for a dog of 10 

 kilos. One-half of a cubic centimeter of this warm solution is then 



a Josserarid, P. Contrib. a Tetude physiol. de l'adrenaline. These. Paris. 1904. p. 31. 



b Abel. J. J. On a simple method of preparing epinephrin and its compounds. Bui. 

 Johns Hopkins Hospital, vol. 13. p. 30. 1902. Prof. Abel says: "I judge from 

 the bulkiness of the phosphomolybdate precipitate that a quantitative experi- 

 ment would result in a high percentage of impurity. * * * Commercial adre- 

 nalin was * * * purified * * - and more than thirty analyses of various 

 fractions have been made, but it has been found impossible to secure uniformity 

 of composition among the various products."* 

 Function of the suprarenal glands. Contributions to Med. Research, dedicated 

 to V. C. Yaughan. p. 155. 

 Compare also Gurm. A., and Harrison. E. F. A new characteristic reaction of 

 adrenaline. Pharm. Jour.. 1907. p. 718. 



c Abderhalden. E.. and Bergell. P. Munch. Med. Woch., vol. 51. p. 1003. 1004. 



* Takamine says "100 parts of adrenalin needs nearly 19 parts of hydrochloric acid 

 in forming a neutral salt.'" (Amer. Join. Pharm.. vol. 73. p. 527. 1901. 

 112 • 



