6 
into requisition on a number of occasions to determine actual breeding 
points in mosquito-infested regions, and interest in the subject grad- 
ually increased until, during the past two or three years, the researches 
of those medical men, whose names have since become so well known 
in this connection, showed by exact methods that Dr. King’s theory 
must no longer be considered a theory but a demonstrated fact. It 
has resulted that the attention of the entire civilized world has been 
drawn with vivid interest toward the whole mosquito question. Every 
fact concerning mosquitoes becomes now of great potential importance. 
The correspondence of this office on mosquitoes, owing largely to its 
publications, has become greatly increased. The writer has been 
invited to address scientific bodies and citizens’ improvement associa- 
tions on the subject of mosquito extermination, and in the spring of 
the present year lectured before the annual meeting of the Royal 
Society of Canada and before the section on theory and practice of 
medicine of the American Medical Association on the subject of the 
biology of the mosquitoes of the genus Anopheles as contrasted with 
that of the mosquitoes of the genus Culex. The demand for the pub- 
lications of this Division on mosquito subjects has been so great that 
it has been deemed desirable to bring together the published and 
unpublished articles and notes in convenient reference form. from the 
standpoint of the United States only, and this has been done in the 
present bulletin. 
The writer is indebted to his assistants, Mr. D. W. Coquillett, for 
determinations of the different mosquitoes discussed; Mr. F, C. Pratt, 
for untiring efforts in the collection of material; Mr. August Busck, 
Mr. R. 8S. Clifton, and Mr. J. Kotinsky, for assistance in laboratory 
experiments, and Miss L. Sullivan, for the preparation of the illustra- 
tions. Information and specimens derived from many correspondents 
are acknowledged in the pages of the bulletin. 
1 QOoEs 
