ARTIFICIAL STIMULATION TO PROLIFERATION. 31 
RESULTS WITH SQUARES. 
As in previous tables the records for squares and bolls are kept 
separate. 
TaBLe X.—Results of experiments at Hidalgo, Tex., in producing proliferation in squares 
by artificial stimulation. 
| 
| 
2 = } - Weevil Effects of | % B® 
a 3 | o stages artificial | == 
= Se found. punctures. 2% 
Z - | 2 > 3} seo = —— = 
) = Sy C=Hhey Pese= J 1 = be ra oe 
: =S8/55|/52| *3 |s ee 5 Lop 
on => < So a os mt Oss LS - 
Treatment of squares. Ss }og|/3e| gf |S.1 a8 |Sel55 |] e838 
weiss |Fs| 8S | fs |)es | 39128 fees 
Dike he oe me Se a2f\12e\5a lee aes 
2 SE Oss Pears S 2 |Sn lsolaesaioga 
= = ae a> As |i Ge =z =A oS 
=| ne} re > a5 "yen =aa\5s nee 
= iS) ° 3 Ss rae | PE i! 5 ee 
Z = = A = = Ay Ay A, 
ED (tae (Se eee eee eee Meee hey 
- = cos | 
Puncture made with No. 12 sterilized nee- 
Pee MINSERCO ie - chen oe ore oa once ee 5 5 1 12.8 if 0 5 0 100.0 
Puncture made with No. 12 sterilized nee- | 
dle; sealed with shellac..........-.-...- 5 | 5 1 13.4 1 0 0 5 0.0 
Puncture made with hypodermic syringe 
needJe, sterilized; unsealed.........--.-- 4 . 2 14.0 2 | 0 0 4 0.0 
Puncture made with hypodermic syringe | 
needle, sterilized; sealed with shellac. - rg) ial 2 12.7 2 0 2 | 5 28.6 
| 
Puncture made with hypodermic syringe | | 
needle; one-half drop of 2 per cent solu- | 
tion of formic acid injected; unsealed .- . 3 | 3 0 14.0 0) 0 0 | 3 
Puncture made with hypodermic syringe 
needle; one-half drop of 2 per cent solu- 
tion of formic acid injected; sealed with 
SSG AOU Sree ee ae See ee eee ee 10 10 Sci 13.4 4 | 0 5 5 
Puncture made with hypodermic syringe | 
needle; one-half drop of 2 per cent solu- 
tion of caustic potash injected; unsealed. a 
Puncture made with hypodermic syringe 
needle; one-half drop of 2 per cent solu- 
tion of caustic potash injected; sealed | 
| 
| 
| 
| | 
MAIO MONRE So 8252> 38 tds nae oka os 5) 5| 3 10.2 3 filt.23 2 | 60.0 
bo 
to 
Totals and averages ...............- 43 43 16 13.0 14 1); i7| 2% | a 40.0 
a Weighted average. 
It must be remembered that in all cases these artificial punctures, 
though sealed, resemble feeding punctures of the weevil much more 
closely than they do egg punctures. It is impossible to imitate 
artificially the natural conditions following oviposition, the hatching 
_ of the egg, and the gradually increasing irritation accompanying 
the growth of the larva. Really the effect of a needle puncture 
upon the tissues penetrated is very different from the feeding punc- 
tures of the weevil. The needle simply crushes the cells, pushing 
them aside from its path, and leaving the cavity it makes more or 
less completely filled with sap and crushed ¢ells. In the weevil 
puncture the work is far more neatly done than it can be in any 
bungling imitation. The sharply toothed mandibles at the tip of 
_ the weevil’s snout cut away the tissues smoothly without crushing 
_ or injuring adjoining cells, and the material, being eaten, is entirely 
_Temoved from the cavity, leaving it dry and clean with the adjoining 
tissues comparatively uninjured. The difference is really about as 
great as in a case of the accidental amputation of a limb under a 
railway train as compared with the work of a skilled surgeon. The 
