- THE CAYENNE TICK. 155 
The larve (Tables LXVII, LXVIIT).—The greatest longevity of 
larve observed by us was 386 days: These larve hatched June 
24, 1908, and were kept in a tube on moist sand in the laboratory. 
As is shown in Table LXVII, the greatest longevity out of doors 
was 368 days.. In many cases we have observed a few larve to be 
alive over a year after the first eggs deposited by the parent had 
hatched. The minimum period required for engorgement was 3 
days, the greatest number dropping on the fourth day. Newstead 
states that on man engorgement is completed in from 2 to 4 days. 
TasLE LXVIII.—E£ngorgement of larve of Amblyomma cajennense on bovine. 
Total 
Date larvz applied. number 
ee eae eee TS | 7 | aia | es = 
wey oe: LOOT 2 P33) oP 0 0 20 63 9 0 0 0 0 0 92 
PRG SO on a 0 0 0 56 40 3 0 0 0 0 99 
Mn 24, 1908. RE 0 0 0 12 17 30 + 0 0 0 63 
cy, i a 0 0 o| 2! 2 0 0 0 0 0 44 
cig CSE |S ee ee eee 0 0 0 18 64 0 0 0 0 0 §2 
LE ig 1 Aa ie ae ee ee 0 0 0} 185 77 20 0 0 0 0 282 
Spee NG et, ee 0 0 0 0 8] 17 0 0 0 0 25 
A few days after dropping the larve cluster together, as ln ameri- 
canum, and become quiescent. Soon the anterior part of the body 
becomes light colored, showing that metamorphosis is taking place. 
The shortest period in which molting took place was (at a mean tem- 
perature of 89.8° F.) 10 days. In this instance a total effective tem- 
perature of 468° F. was accumulated. All of the larvz observed were 
engorged on bovine animals except three lots which were engorged 
on guinea pigs. 
The nymph (Table LXIX).—In order to determine the longevity 
of nymphs, 20 that molted July 29, 1907, were isolated in a tube. On 
November 13, 1907, all were alive; on March 15, 1908, 15 remained 
active; on July 12, 12 were alive, and on July 29, 8 were alive. On 
September 5, 1908, 5 were still alive, after a period of 1 year and 43 
days. Another lot of 40 nymphs which molted from larve September 
29 to October 5, 1908, contained 1 individual which lived until June 
21, 1909, a period of 265 days. Ina third lot which became nymphs 
September 28, 1908, 5 specimens were alive after a period of 269 
days. 
The shortest period in which engorgement took place was 3 days, 
the largest number dropping on the fourth and fifth days. 
