16 MISCELLANEOUS PAPERS. 



symptoms appeared, the rabbit weighing 4 pounds 5 ounces (1,956 

 grams). After receiving three further doses, representing 200 gram- 

 each, in a period of five days the animal showed no symptoms of 

 " trembles " or of suffering of any sort, the weight increasing under 

 this treatment to 4 pounds 8 ounces (2,041 grams). The animal which 

 had received this treatment gave normal delivery to young and made 

 a rapid recovery, the young appearing abnormal in no respect. Thus. 

 although the rabbit received the equivalent of 1.000 grams of the 

 plant in a week, no symptoms of milksickness appeared and the 

 animal steadily gained in weight. 



After a rabbit weighing 4 pounds (1,814.3 grains) was injected 

 subcutaneously in the back with an extract of 20 grains of the dried 

 plant muscular tremors were felt in the limbs and in the masseter 

 muscles, but recovery followed. 



When a quantity of the same extract representing 40 grams was 

 injected into a rabbit weighing -1 pounds 1 ounce (1,842.7 grams) 

 no results followed, but an extract of 60 grains of the dried plant 

 proved fatal in about twelve hours, the animal showing distinct 

 tremors. 



Following the injection of an aqueous extract representing 5 grams 

 of the dried plant into a rabbit weighing 3 pounds 8 ounces (1,587.5 

 grains) there were no tremors and the rabbit was apparently normal, 

 though the temperature rose 2.2 degrees F. in two hours and twenty- 

 five minutes after the injection. 



A rabbit weighing 3 pounds 12 ounces (1.700.8 grams) was in- 

 jected subcutaneously with a concentrated extract representing 10 

 grams of dried Eupatorium and no tremors resulted, but in the two 

 and one-half hours following the injection a fall of one-half a degree 

 in the temperature was noted. Death followed during the night. 



On increasing the quantity of extract until it represented 20 grams 

 of the dried plant and injecting this subcutaneously. slight tremors 

 were felt on careful examination by the hand over the hips after 

 almost an hour and a half. An hour and forty minutes later no 

 tremors were felt and the rabbit seemed normal. The temperature 

 two and one-fourth hours after injection fell 2 degrees F. Death 

 ensued during the night. 



These animals had been kept under observation for two weeks pre- 

 ceding the experiment and had shown nothing abnormal. 



EXTRACTS FROM FRESH PLANTS PRESERVED IX CHLOROFORM. 



To prepare extracts from material preserved in chloroform the fresh 

 plant was placed in a container and covered with water containing 

 enough chloroform to prevent fermentation. "When wanted for use 

 the liquid was pressed out of the plant and concentrated in vacuo to the 



121—1 



