103 



The sample was reduced to the stock solution of 1 to 100, and a jar 

 with 500 cc of water was stocked with larvae and pupa? of Oulex and 

 Anopheles. At 9.25 a. m. 1 added 5 cc of the stock solution. At 10.10 

 a. m. the Oulex larvae were dead; at 10.30 the Anopheles began to die, 

 and all were dead at 2 p. m. All pupa? were yet alive. Next morning 

 6 adults were found in the hood and 5 cc of the stock solution was 

 added, doubling the amount in the water. Yet twenty-four hours 

 later several adults were again in the hood and all the pupa? were 

 alive, seeming not in the least discommoded by the disinfectant. 



The difference between the action of the material on the larvae and 

 on the pupae is most remarkable and, once the pupal stage was reached, 

 development seemed not in the least interfered with. 



The sample was reduced to the stock solution of 1 to 100 and two 

 jars with 500 cc of water were stocked with larvae and pupae of Ano- 

 pheles and Oulex. At 4.30 p. m. I added 5 cc of the stock solution to 

 jar No. 1, and at 4.55 most Oulex and some Anopheles larvae were 

 dead. At 5.30 all Anopheles were dead, but a few Oulex larvae were 

 yet alive. Next da} T , at 8 a. m., a few pupae remained alive; at 12 m. 

 everything was dead. 



To jar No. 2 I added 10 cc of the stock solution at 4.30 p. m. At 

 4.50 all Oulex larvae were dead; at 5.30 only pupae remained alive; 

 next morning everything was dead. 



SOLUBLE BLAST FURNACE OIL. 



I reduced the sample to the stock 1 to 100 mixture and stocked two 

 jars, each containing 500 cc of water, with larvae and pupa 1 of Oulex 

 and Anopheles. At 3.30 p. m. I added 5 cc of the stock to jar No. 1, 

 and in twent}^ minutes all Oulex larvae were dead. At 4.40 p. m. all 

 the larvae were dead, and though the pupae were alive, the adults died 

 as they attempted to emerge. 



In jar No. 2 1 added 10 cc of the stock at 3.25 p. m. All larvae 

 were dead in five minutes; but at 5.30 some pupae wore yet feebly 

 alive. 



Of the phinotas preparations nothing is equal in effectiveness to the 

 " phinotas oil." The other preparations mix fairly well with water, 

 form no surface scum, and all impart a milky tinge to the water. The 

 proportion where 5 cc of the stock was used is 1 to 10,000; where 10 

 ce was used is 1 to 5,000. 



