107 



pupated and some of the pupa? had transformed to adults. I added 

 10 cc from the stock, but when, three hours later, no additional effect 

 Was noted, the experiment was closed. 



The usual 1 to 100 stock was prepared and 5 cc was added to 500 cc 

 of water containing larvae and pupae of Culex pungens. Two days 

 later nothing had been killed and many adults had issued. I added 

 10 cc from the stock, and when, two hours later, no added effects were 

 noticeable, added 10 cc more. After waiting yet twenty-four hours 

 and when nothing had been killed, 1 considered myself fairly entitled 

 to class this as a harmless material. 



This is one of the materials entering into the preparation of synol 

 soap, and it was reduced to the usual stock solution. 



To jar No. 1 was added 5 cc to 500 cc of water containing larva? and 

 pupa? of Culex jnmgens, at 9.15 a. m. At 12 m.. no effect appearing, 

 I added 15 cc of stock, which killed some larva? by 5 p. m. 



To jar No. 2 was added 10 cc to 500 cc of water as above. At noon 

 no bad effects were noticeable on the larva?. At 1.20 p. m. I added 

 30 cc of stock, and at 5 p. m. only one pupa remained alive. 



This is not a reliable larvicide, and is as expensive as it is ineffective, 



"sample c, j. & J." 



This was reduced to the usual stock of 1 to 100, and at S a. m. 5 cc 

 was added to 500 cc of water containing larva? and pupa? of OuU x and 

 Anopheles. At 10.30 adults began to issue and, as neither larva? nor 

 pupa? seemed affected, I added 5 cc from the stock. At 1 p. m. 

 nearly all Culex and some Anopheles larva' were dead. Next morning 

 all pupa? were yet alive and several adults had issued. I then added 

 20 cc from the stock, but three hours later not even one more pupa 

 had died and the culture was closed. 



" SAMPLE T.j J. & J." 



This was reduced to the usual stock solution. 1 to 100, and 5 cc was 

 added to 500 cc of water containing Larvae and pupse i^( Cvlex and 

 Anophdes. No effect being apparent three hours later. I added L5 cc 

 from the stock, which killed all larvae in one and one-half hours. 

 Twelve hours later everything was dead. 



In another jar I added 10 c. c. of the stock, and all save a few mature 

 larva 1 were dead in live hours. Twenty-four hours later a few of the 

 larva' and all the pupa? were yet alive. 



