found on the roadside about a mile from town some post-oak sprouts 

 on which Phylloxera abounded in all stages of development. The pale 

 smooth variety was most numerous on the older and tougher leaves, 

 while the darker, tubereled larvae and pseudo-pupae and a few winged 

 specimens crowded the unfolding second growth. The young leaves 

 were much curled and distorted by their innumerable punctures. The 

 aphids were preyed upon by the larvae of the green Gkrysopa and of 

 one or two small Coccinellids ; the deep red larvae of a small Thrips was 

 especially active and numerous among them, as also was the whitish 

 gray larva of a small bug (No. 3 of my consignment of October 2). 



Put up specimens on slides and sent some alive to Mr. Howard in 

 tubes ; also some in alcohol. I also placed winged individuals in three 

 different sizes of tubes and on clean leaves in water in a glass jar. 



August 3. — Notwithstanding all my care all the winged lice perished 

 without leaving any eggs, to my great disappointment. Probably the 

 extreme heat of the weather was unfavorable. Cleaned and disinfected 

 my tubes and put in fresh winged forms and closed the tubes with loose 

 cotton instead of cork to prevent excess of moisture. 



August 3. — Another failure. All the specimens dead and molded 

 and no eggs. I can not understand it. 



August 15. — Made another excursion out into the country and suc- 

 ceeded in obtaining a very few. Nearly all the young oaks so badly 

 infested two weeks ago are entirely cleared, probably by the migration 

 of the winged forms and the destruction of eggs and wingless forms by 

 predatory insects. The tender shoots are also killed and stand up stiff 

 and dry ; the drought being severe, there was no opportunity for them 

 to recover from the attacks of the aphis. 



On the lower mature leaves the insect still abounds in its wingless 

 forms, mostly the flat, pale, smooth variety. Put seven of the winged 

 aphis into a very small tube with a bit of mature oak-leaf and closed it 

 tightly with common cork. (The rubber corks kill all insects inclosed 

 with them very shortly, probably with their sulphurous emanations.) 

 Also put about a half dozen on growing sprouts of oak, out of doors, 

 but which seemed to be entirely free from infection, inclosed under fine 

 muslin cover. 



August 17. — Have at last succeeded in getting nine eggs in the small 

 tube. These eggs do not differ much from those of the ordinary apter- 

 ous form. On close comparison they seem rather more opaque and of 

 a deeper yellow tint than the latter, but even in these particulars there 

 is some variation. Each individual produces from one to three eggs; 

 and usually perishes beside them. 



August 21. — Eggs still healthy and beginning to show segmentation 

 with two red eye-spots on the cephalic end. There is evidently a slip- 

 ping backward of the thin pellicle that incloses them, although as yet 

 no motion is discoverable. The eggs were all laid on the glass and not 

 on the portion of the leaf inclosed with them. 

 August 22.— Three Phylloxera have hatched from the eggs and one 



