46 PAKASITES BELONGING TO THE GENUS GLOMEEELLA. 



and Rolfs (66) have referred the conidial form of the fungus on 

 avocado to Colletotri'chum gloeosporioides and Bessey (12) reports 

 successful cross-inoculations from citrus fruits and mangos, thus 

 confirming its identity physiologically as well as morphologically 

 with the organism on the orange, which in turn can not be satisfac- 

 torily separated from the form on privet, apple, etc. According to 

 the authors cited, infection takes place through the flowers. Higgins 

 (47) has recently described a serious disease of avocado in Hawaii 

 which he considers as probably due to Gloeosporium. Its effect upon 

 the plant, attacking as it does flowers, foliage, and shoots, is the 

 same as that of the organism just described. 



PHASEOLUS VULGABIS L. (WAX BEAN). 



Glomerella lindemuthianum Shear, n. comb. 

 Colletotrichum lindemuthianum Sacc. and Magn. 



Numerous cultures of the bean anthracnose have been made at 

 different times and in different seasons. The cultures from conidia 

 in most cases have been rather uniform in appearance and behavior, 

 agreeing with the descriptions given by Atkinson (3), Whetzel (95), 

 Edgerton (30), and others. In 16 slant agar tubes made November 

 3 from different acervuli on 5 bean pods the growth soon became very 

 dark colored. This appears to be quite a constant characteristic of 

 this species, and all the cultures made were practically identical in 

 appearance during their growth. No perithecia were produced in 

 these cultures. 



The acervuli varied greatly in number and size in different cul- 

 tures, and setae, though usually present, were not numerous. Many 

 chlamydospores were also found in some cultures. Cultures from 

 single conidia, showing the usual appearance of the fungus, are 

 shown in Plate VII. 



In December cultures were made in flasks of corn meal by transfer 

 of conidia and a bit of tissue from a bean pod bearing acervuli of the 

 bean-anthracnose fungus. Later, all of the flasks showed good peri- 

 thecia and asci, but conidia were scarce or wanting. An ascus and 

 ascospores are shown in Plate I, figures 13 and 13a. These cultures 

 finally became contaminated and were discarded. Plates were pre- 

 viously made from them, using the crushed perithecia and asci. 

 The ascospores germinated readily and produced a dense growth of 

 mycelium of the usual appearance. No conidia were found in these 

 plates. Other plates poured from the same ascospore material pro- 

 duced the same typical mycelial growth; and at the end of 12 days 

 perithecia were present in great numbers, but no conidia were found. 

 Other plates were poured on March 16. A single ascospore trans- 

 ferred to a tube and afterwards to a flask of corn meal produced the 



252 



