50 PARASITES BELONGING TO THE GENUS GLOMERELLA. 



On April 30 two small young leaves in whose axils no flower buds 

 were located, also two leaves a year old in whose axils fruit had been 

 borne, were treated as usual and placed in moist chamber. On May 

 16 acervuli were abundant on the old leaves, but none were present 

 on the new leaves. Though kept until June 22 no acervuli or perithe- 

 cia developed on the young leaves, while acervuli and perithecia were 

 numerous on the older leaves. This would suggest the possibility 

 of the fungus having entered the old leaves by way of the flowers of 

 the previous season, while the flowers in the axils of the new leaves 

 not having opened, the new leaves had not yet become infected. 



One leaf, in the axil of which fruit had just set, did not develop 

 any Gloeosporium in moist chamber nor did another which bore a 

 flower in its axil. 



Four terminal leaves from different shoots situated 1 to 4 inches 

 beyond any fruit and four leaves at the base of fruits, were placed in 

 moist chamber. One leaf in each set developed acervuli. These 

 last experiments do not seem to indicate any clear connection between 

 flowers and fruit and infected leaves in this case. It seems more 

 probable that the dormant infections came from local infection of the 

 leaves. 



CULTURES. 



Numerous cultures started from conidia from leaves placed in 

 moist chamber at different times during the year, produced a mycelial 

 growth of the usual general appearance of Glomerella. Acervuli 

 were formed in all the cultures, but usually they were not numerous. 

 The spore masses were pink. No setae were found in any of the cul- 

 tures. Chlamydospores occurred in some. Perithecia were never 

 found in the cultures. 



Cultures made from conidia from fruit of guava in the greenhouse 

 had the same general appearance as those from leaves. Setse ap- 

 peared, frequently in great abundance, in nearly all the cultures 

 derived from fruit. Chlamydospores were also abundant in some of 

 these cultures, but no perithecia were found. The conidia from the 

 host ranged from 13.5 to 19.5 by 4.5 to 6 fi. Conidia from cultures 

 varied from 13.5 to 18 by 4.5 to 6 /*. Ascospores from the host 

 ranged from 13.5 to 21 by 5.5 to 6 /*. An ascus and ascospores are 

 shown in Plate I, figures 9 and 9a. 



There is nothing, so far' as could be determined, in the morphological 

 characters or behavior of this fungus in cultures or on its host to 

 distinguish it from Glomerella cingulata as it occurs on citrus fruits 

 and other evergreen-leaved plants as well as pomaceous fruits. 

 Inoculation experiments reported by Cobb (21) indicated that the 

 fungus could be successfully inoculated into apple, banana, pear, and 

 quince. The form on apple was also successfully transferred to 

 guava by the same investigator. 



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