STUDIES OF GLOMERELLA FROM DIFFERENT HOSTS. 53 



these cultures. Other specimens of the same diseased dewberries 

 kept in moist chamber also produced acervuli. Poured plates were 

 made with conidia from these acervuli. Acervuli but no setae were 

 found in the cultures, and no perithecia were produced. The conidia 

 varied in size from 9 to 16 by 4.5 to 6 y.. In one set of these cultures 

 the conidia averaged rather smaller than in the others, being mostly 

 9 to 13.5 by 4.5 /*. This form does not appear to be distinct from that 

 just described from the black raspberry, though the material used in 

 these cultures produced no perithecia. Inoculation experiments 

 described later show that this form adapts itself quickly to the apple. 



SMILAX MEDIC A SCHL. AND CHAM. 



Gloeosporium rufomaculans (Berk.) TMm. 



On October 31 acervuli of a Gloeosporium were found on leaves 

 of this Smilaxin the greenhouse of the Department of Agriculture. 

 Plates which were poured from this material produced a growth of 

 mycelium of the usual character and an abundance of conidia but no 

 distinct acervuli. Transfers were made from these plates to tubes 

 which soon produced large acervuli with pinkish masses of conidia. 

 Later, the mycelium became dark colored, but no perithecia were ever 

 found and no setse were seen. The conidia ranged from 10.5 to 

 19.5 by 5 to 6 y.. The fungus as it appeared on the host and in 

 cultures showed no characters to distinguish it from the conidial 

 forms of Glomerella found on most of the other hosts studied. No 

 species of Gloeosporium or Glomerella seems to have been reported 

 heretofore on Smilax. 



THE A JAPONIC A (l.) BAILL. (CAMELLIA). 



Glomerella cingulata (Stonem.) S. and v. S. 

 Colletotrichum camelliae Mass. 



DEVELOPMENT ON LEAVES IN MOIST CHAMBER. 



On January 29 apparently normal, healthy leaves were taken from 

 a greenhouse plant, the surfaces sterilized as usual, and the leaves 

 placed in a sterile moist chamber. They soon began to show a dark 

 discoloration extending from the petiole up the midrib almost to the tip 

 and finally became entirely discolored. Acervuli occurred on all the 

 leaves, and fertile perithecia of Glomerella were also present but not 

 abundant. 



CULTURES. 



Tube cultures were made, using conidia from the acervuli on the 

 leaves in moist chamber, as described above. These cultures pro- 

 duced abundant acervuli but no perithecia. No setae occurred, 

 though setae were abundant in the acervuli on the leaf. In other 



252 



