STUDIES OF GLOMERELLA FEOM DIFFERENT HOSTS. 55 



acervuli. Mature perithecia of Glomerella were found in both these 

 cultures in about three weeks. Chlamydospores were also present. 



On July 5 a tube culture was started from a single conidium. This 

 developed the usual growth and produced acervuli. Two subcul- 

 tures were made in flasks of corn meal. These cultures started about 

 as usual and were identical in appearance, but the mycelium began 

 to assume a bluish smoky color rather sooner than in most cases. 

 Acervuli with pinkish masses of conidia also developed. These cul- 

 tures were kept for nearly a year, but no perithecia were ever found. 

 The conidia on the leaves in moist chamber ranged from 15 to 21 by 

 4.5 to 6.5 fx. 



Several species of Gloeosporium and Colletotrichum have been 

 described as occurring on tea. They all appear to belong to this 

 Glomerella except Gloeosporium theae-sinensis Miyake which has 

 conidia only 4 to 6 by 2 ji. The conidial form might be referred either 

 to Gloeosporium or Colletotrichum, as setae were found on leaves in 

 moist chamber but not in cultures. A microscopic study of the type 

 specimen of Massee's (58) species, Colletotrichum camelliae, leaves no 

 doubt of its identity with the conidial form of the fungus just 

 described. 



THEOBROMA CACAO L. (CHOCOLATE NUT). 



Glomerella cingulata (Stonem.) S. and v. S. 

 Colletotrichum theobromicolum Delacr. 



On January 19 leaves of cacao or chocolate nut showing small dead 

 areas but no fungus fructifications were taken from greenhouse plants, 

 the surfaces sterilized as usual, and placed in a moist chamber. 

 Many acervuli of Colletotrichum soon appeared, especially along the 

 midrib. Setae were abundant and also immature perithecia, which 

 later (March 10) showed an abundance of mature asci and ascospores. 

 Paraphyses were also seen. An ascus and ascopores are shown in 

 Plate II, figures 24 and 24a. 



Plate cultures were made, using conidia from the leaves in moist 

 chamber just described. Transfers were made from these plates to 

 four tubes in which a growth of whitish mycelium of the usual char- 

 acter developed and soon large acervuli with pinkish masses of 

 conidia appeared at the point of planting in two of the tubes. In 

 the other two no distinct acervuli were found. Setae were more fre- 

 quently present on the host than in the cultures. In some of the 

 poured plates no setae were found. In one tube where setae were 

 abundant one was found bearing a spore, as sometimes happens in the 

 species occurring on cotton. No perithecia were ever found in any of 

 these pure cultures, and no ascogenous fungus has been described 

 from this host, so far as noted, which seems to agree with the 



252 



