PEDIGREED CULTURES OF G. CINGULATA FROM AVOCADO. 59 



CONIDIAL GENERATIONS FROM THE SAME HOST, 



Generation 1 was started from a single conidium taken from a 

 culture which also produced fertile perithecia. Acervuli and conidia 

 were abundant at first in these cultures and a few perithecia formed 

 in the lower part of the tubes. 



Generations 2, 3, and 4 varied but slightly in general appearance 

 and relative abundance of acervuli and perithecia from generation 1. 



Generation 5 was practically the same as generation 4. 



Generations 6 and 7, consisting of three tubes each, showed acervuli 

 in abundance and lines of submerged perithecia developed in the 

 medium as in some of the ascospore cultures, but they were much 

 fewer. 



Generation 8 showed an apparent reversion to the condition of the 

 first five generations, with the same general distribution, arrangement, 

 and relative number of acervuli and perithecia. 



Generation 9 was strikingly different from generation 8. Acervuli 

 were few and the perithecia very numerous and thickly scattered 

 over the surface of the medium, resembling most of the ascospore 

 generations. Tube 9a is shown in Plate IX. Two plates poured 

 from this tube showing the same predominance of perithecia are 

 illustrated in Plate XIV. 



Generation 10 showed an apparent reversion again to the form in 

 the first five generations. Acervuli were abundant and large; the 

 perithecia were fewer and mostly grouped about the acervuli. 



Generation 11, tube b, was practically identical in appearance with 

 generation 10, tube b. Acervuli and perithecia were numerous. 

 Generation 11, tube c, was very strikingly different from generation 

 10, tube c, showing large acervuli and masses of conidia, but very 

 few perithecia. 



Generation 12, consisting of tubes b and c, did not show the char- 

 acters of the previous generation but rather the reverse, perithecia 

 being abundant and acervuli few. 



Generation 13 produced abundant acervuli, but the perithecia 

 were much fewer than in generation 12. The two tubes b and c are 

 shown in Plate VIII. 



Tubes b and c of generation 14, derived from generation 13, tubes 

 b and c, respectively, were strikingly different. Tube b produced 

 almost entirely conidia with scattered acervuli, while c consisted 

 almost entirely of perithecia formed along the line of inoculation. 

 These tubes are shown in Plate VIII. 



Tubes b and c, generation 15, were very similar to tubes b of gen- 

 erations 13 and 14. A few perithecia were present in each, but they 

 were not numerous and predominating, as in tube c of generation 14. 



252 



