32 PAEASITES BELONGING TO THE GENUS GLOMEEELLA. 



were present, but a few mature perithecia of Glomerella were found. 

 Later, acervuli also developed. 



On July 12 another apparently normal healthy leaf was treated in 

 the same manner. On July 20 the under surface of the leaf had be- 

 come light chocolate brown and was thickly covered with minute 

 acervuli. Some larger, brighter colored ones were found on the petiole 

 and about the midrib. No perithecia were found on this leaf. Many 

 other leaves of this host have been treated in the same manner at 

 different times, usually producing both conidia and ascospores. 



CULTURES. 



On February 11 streak agar tube cultures were made, using conidia 

 from a leaf in moist chamber. On April 8 perithecia were abundant 

 in one tube, being mostly aggregated in masses about the bases of 

 old acervuli. Appressoria were also abundant on the surface of the 

 glass in the upper part of the tube. Subcultures on corn meal in 

 flasks produced an abundance of fertile perithecia also. 



On April 1 bits of leaf bearing mature perithecia with ascospores 

 were transferred to flasks of corn meal. On April 26 an abundant 

 growth of conidia and also mature perithecia were found. Setse 

 were sometimes found in the cultures but not regularly. 



Koorders (54) has investigated this form of the fungus as it occurs 

 in Java. He refers the perithecial form to a new genus, Neozimmer- 

 mannia. There is, however, nothing in his description to separate 

 his fungus from Glomerella and specimens of our plant submitted 

 to Dr. Koorders for examination were said by him to be identical 

 with his fungus. The conidial form has been called Gloeosporium 

 elasticae Cke. and Mass. It can not be distinguished from the forms 

 found on other Ficus spp. The asci were found to vary from 52 to 

 82.5 by 7.5 to 12 p. Plate II, figures 18 and 18a, shows an ascus and 

 ascospores. 



FICUS LONGIFOLIA-SCHOTT. 



Glomerella cingulata (Stonem.) S. and v. S. 



DEVELOPMENT ON LEAVES IN MOIST CHAMBER. 



On November 18 two leaves of Ficus longifolia showing small dead 

 areas were taken from a greenhouse plant, treated as usual, and placed 

 in a sterile moist chamber. On December 6 numerous acervuli 

 were present, producing masses of salmon-colored conidia. No 

 setse were found at this time. On January 4 setse were present and 

 also perithecia with mature asci and ascospores. 



On January 29 another apparently healthy, normal leaf was taken 

 from a greenhouse plant, sterilized as usual, and placed in moist 

 chamber. On February 15 the leaf showed discolored spots of a 



252 



