STUDIES OF GLOMEEELLA FEOM DIFFEEENT HOSTS. 29 



cultures varied from 10 to 16.5 b}^ 4.5 /i. The ascospores from the 

 leaves in moist chamber showed the usual shape and varied from 

 15 to 21.5 by 5 to 6 fi. 



CEATAEGUS SP. 



Gloeosporium fructigenum Berk. 



On December 12 three flasks of corn-meal agar were inoculated 

 with spores from acervuli on a fruit of Crataegus which had been 

 collected from a tree on the Department of Agriculture grounds 

 and kept in moist chamber. The appearance of the acervuli and 

 the decay of the fruit closely resembled that of the bitter rot of the 

 apple. Conidia developed in these cultures and small dark bodies 

 resembling perithecia were found in all the flasks, but no asci or 

 ascospores were obtained. 



CUCUMIS SATIVUS L. (cUCUMBEE). 



Gloeosporium lagenarium (Pass.) Sacc. and Roum. 



On June 20 four plates were poured, using conidia from a cucumber 

 leaf collected at Portsmouth, Va. Two days later transfers were 

 made to slant agar tubes. On July 6 the tubes showed an abun- 

 dance of acervuli with a dense growth of brown setse. The cultures 

 became contaminated and no perithecia were formed. No oppor- 

 tunity was offered for a further study of this form and its identity 

 with the others described is therefore uncertain. 



CUCUEBITA PEPO L. (SQUASH). 



Gloeosporium lagenarium (Pass.) Sacc. and Roum. 



On October 17 four plates were poured, using conidia from acervuli 

 on a squash. The conidia and acervuli were of the same general 

 appearance as those from other hosts. Setse were abundant. On 

 October 22 conidia were abundant in all the plates, but were scat- 

 tered, no distinct acervuli being formed. The conidia were un- 

 usually variable in size, ranging from 8 to 30 /i in length. In the thickly 

 sown plates dark bodies suggesting perithecia were abundant. None 

 of these dark bodies were present in the thinly sown plates. These 

 peritheciumlike bodies always remained sterile. Chi amydosp ores 

 were also abundant. The mycelium was light colored. Later, many 

 acervuli with pale pink masses of conidia developed in the tubes, and 

 also an abundance of the sterile perithecia. The cultures were kept 

 until January 13, but no ascospores were ever found in the perithecia. 

 The writers' cross-inoculations of squash with the fungus from the 

 grape were successful, but this does not necessarily prove that the 

 organisms are the same. No inoculations from the squash to other 

 fruits were made. 



252 



