28 PAEASITES BELONGING TO THE GENUS GLOMEEELLA. 



lated by transfer of conidia from the tube culture from the single co- 

 nidium mentioned above. On January 20 an abundant greenish 

 growth was present , and many dark bodies resembling perithecia 

 were forming. Many acervuli were also present. No setse were 

 found and no perithecia ever matured sufficiently to show asci and 

 ascospores. 



On June 12 two slant agar tubes were made by transfer from the 

 single conidium culture. These tubes produced a growth of the usual 

 appearance and small acervuli formed, but no perithecia were ever 

 found. 



The growth and appearance of the conidial form from the coffee 

 closely resembles that from other hosts, especially the Citrus species. 

 Setse and perithecia developed on the leaves in moist chamber but 

 neither were found in the cultures made from the same leaves. The 

 conidia from the leaves in moist chamber measured 13.5to 16.5 by 5 

 to 6 ji. The conidia in culture varied from 15 to 18.5 by 4.5 to 6 /*. 

 The ascospores from the leaves (PL I, figs. 14 and 14a) measured 15 to 

 18 by 5 to 6 p.. No paraphyses were found. The perithecial form 

 found on the leaves suggested Laestadia cqffeicola Speg. (86) . The asco- 

 spores of this species are said to be obovate, and the measurements 

 given are rather less than usual in Glomerella cingulata. Two species 

 of Collet otrichum and two species of Gloeosporium have been described 

 from coffee leaves. The descriptions of these species show no diag- 

 nostic characters sufficient to separate them satisfactorily. Gloeo- 

 sporium coffeanum Delacr. (23) evidently refers to this species also. 



COSTUS SPECIOSUS (KOENIG) SMITH (SPIEAL FLAG). 



Glomerella cingulata (Stonem.) S. and v. S. 



On January 30 portions of a leaf showing large dead areas were 

 sterilized as usual and placed in a sterile moist chamber. On Febru- 

 ary 7 acervuli were found on these leaves, and on February 14 many 

 mature perithecia of Glomerella were present. Asci and ascospores 

 are shown in Plate II, figures 21 and 21a. No fungus of this kind in 

 either stage could be found reported on this host. 



On February 7 two tube cultures were made by a transfer of conidia 

 from the leaf in the moist chamber, each culture from a different 

 acervulus. On February 24 one large acervulus was present in one 

 tube and a few large sterile dark bodies in the other. On July 7 a 

 few acervuli were present in the second. The peritheciumlike bodies 

 were still sterile and no asci were ever found. The general appearance 

 of the growth in the tubes was much like that of the fungus from other 

 hosts but somewhat lighter colored. The conidia from the specimens 

 in moist chamber showed the usual variations in shape, and they 

 varied in measurement from 12 to 18 by 4.5 to 6 /*. The conidia in 



252 



