STUDIES OF GLOMEKELLA FROM DIFFERENT HOSTS. 27 



It will be noted that perfect perithecia and asci of Glomerella 

 were frequently produced upon leaves of the various citrus species 

 when placed in a moist chamber. It is therefore somewhat remark- 

 able that in only one case, that of the pomelo, were we able to get 

 perithecia to develop in cultures. 



The fungus from all the citrus hosts showed very similar characters 

 in cultures and can not be distinguished from that on most other 

 fruits. When old the mycelium is usually of a dark greenish or 

 smoky color. The form of the fungus growing on citrus species has 

 apparently been described several times under different names in 

 both its conidial and ascogenous conditions. The conidial form has 

 been treated by Rolfs (66) and others as CoUetotrichum gloeosporioides 

 Penz. (63). To judge from the descriptions and figures, it has also 

 received several other generic and specific names. The conidia have 

 been found to vary from 11 to 19.5 by 4.5 to 6 p.. The ascospores 

 vary from 16 to 19 by 4.5 to 6 fi. 



The perithecial form has apparently been described under several 

 different names, including Physdlospora citricola Penz. It is also 

 found under Laestadia soda Penz. 



COFFEA ARABICA L. (COFFEE). 

 Glomerella cingulata (Stonem.) S. and v. S. 



DEVELOPMENT ON LEAVES IN MOIST CHAMBER. 



On December 4 leaves showing dead spots but no acervuli were 

 taken from a coffee plant in the greenhouse. The surfaces were 

 sterilized as usual and the leaves placed in a moist chamber. On 

 December 30 acervuli and mature perithecia of Glomerella were found 

 on these leaves. Setse were present in the acervuli. 



On January 29 two apparently healthy young leaves of the same 

 age were treated in the same manner and placed in a sterilized moist 

 chamber. On February 13 numerous acervuli were present on a 

 discolored area, which extended downward from the tip of one of 

 the leaves. The other leaf was entirely discolored but no acervuli 

 were present. Later, perithecia of Glomerella were found associated 

 with the acervuli on both leaves. 



CULTURES. 



On December 12 plates were poured using conidia from the leaves in 

 moist chamber, on which perithecia developed later. A single conid- 

 ium was located in one plate and transferred to a tube. On Decem- 

 ber 19 one plate had become contaminated and was discarded. The 

 other contained a growth of the fungus of the usual appearance. 

 Conidia were found. On December 26 acervuli were present in 

 abundance in the tube and also in the plate. No setae or perithecia 

 were found. On January 8 two flasks of corn-meal agar were inocu- 



252 



