26 PARASITES BELONGING TO THE GENUS GLOMERELLA. 



On May 28 cultures were made in five flasks of corn meal by trans- 

 planting a portion of the discolored epidermis, the so-called "tear- 

 stain," of a lemon received from Bonita, Cal. The surface of the 

 lemon had been sterilized by thorough washing with corrosive- 

 sublimate solution. Appressoria or clilamydospores of Gloeosporium 

 were found on the surface of the discolored skin. On June 2 the five 

 flasks apparently contained pure cultures of Glomerella and were 

 identical in appearance with those made in the same way from pomelo. 

 Conidia were abundant, but no setae were seen. 



In discussing the diseases of the lemon caused by this fungus in 

 California, Essig (35) states that the most satisfactory method of 

 controlling them yet tried is by spraying with 4-4-50 Bordeaux 

 mixture. This statement also accords with the results of Rolfs (66). 



CITRUS XOBILIS LOUR. (MAXDARIX). 

 Glomerella cingulata ( Stonem.) S. and v. S. 



DEVELOPMENT OX LEAVES IN MOIST CHAMBER. 



On January 29 four apparently healthy leaves from greenhouse 

 plants were treated as usual and placed in a sterile moist chamber. 

 On February 11 acervuli of Glomerella were present on all. having 

 developed first at the base of the midrib. On February 29 a few 

 perithecia with ascospores were found. Perithecia of the same 

 Glomerella were also found on spotted leaves from the same plant, 

 which had been kept in moist chamber. 



CULTURES. 



On March 20 two tube cultures were made from acervuli on the 

 leaves in moist chamber. These cultures produced a growth of normal 

 appearance, with acervuli but no perithecia. On October 7 two plates 

 were poured, using conidia from a leaf in moist chamber. These plates 

 produced an abundance of acervuli. A very few setse were found. 

 Setae had also been found on the leaves from which the cultures were 

 made but they were not numerous. Subcultures from these plates 

 also produced an abundance of acervuli, but no setae were seen and 

 no perithecia ever developed, though perithecia with immature asci 

 were found on the leaves from which the original cultures were made. 



On November 14 subcultures on corn meal were made from the tubes 

 just described. Many large acervuli soon formed. The spore masses 

 frequently ran together and formed pink masses covering about one- 

 half the surface of the medium. Dark spots resembling perithecia 

 were also present but no asci or ascospores could be found. These 

 cultures were kept until the following March, but no fertile perithecia 

 were found and no setae were observed. 



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