STUDIES OF GLOMEEELLA FEOM DIFFEKENT HOSTS. 25 



a few acervuli. On April 7 abundant acervuli and perithecia had 

 developed on all the leaves. 



On April 30 two apparently healthy normal leaves were taken from 

 a greenhouse plant just below an unopened flower bud, and a single 

 leaf also was taken from the old wood lower down on the same branch. 

 These leaves were sterilized as usual and placed in sterile moist cham- 

 bers. On May 14 the old leaf showed discoloration extending from 

 the base of the midrib to within half an inch of the tip, but no acervuli 

 were present. On May 16 the end of the shoot from which the two 

 leaves had been taken was cut off and placed in moist chamber. This 

 shoot bore a young fruit about one-third of an inch in diameter, which 

 had developed since the leaves were removed. The leaf next below 

 these two was also taken and placed in moist chamber. On May 26 

 the old leaf showed acervuli. The two from below the unopened bud 

 were still normal in appearance except for a slight discoloration at the 

 base of the midrib extending up about three-fourths of an inch. The 

 young fruit was also discolored, discoloration beginning at the stigma 

 and working back. No acervuli or perithecia developed on the two 

 young leaves and the fruit. 



Since the foregoing was written, Essig (35) has recommended plac- 

 ing leaves in moist chamber to determine the presence of this fungus 

 in lemon groves. 



CULTURES. 



On December 12 two plates were poured, using conidia from a lemon 

 leaf in moist chamber. On December 17 an abundant growth was 

 present in the two plates, which differed greatly in appearance, 

 though both appeared to be pure cultures. In one the mycelium 

 was colorless and uniformly distributed over the surface showing 

 many minute acervuli. Brown chlamydospores had also formed 

 against the surface of the glass. No setae were found. In the other 

 plate the mycelium was slightly colored and denser, with dark points 

 suggesting the beginnings of perithecia. No spores were yet found 

 in this plate. On January 14 acervuli were thickly and uniformly 

 distributed over the surface of the first plate and a few large acervuli 

 were present in the second plate. Setae were also found in the second 

 plate but none in the first. No perithecia developed in either. 

 Subcultures made from both plates to flasks of corn meal made a 

 growth which was identical in appearance and produced an abund- 

 ance of pink acervuli. No perithecia were found. 



On December 18 four plates were poured, using conidia from four 

 separate acervuli from the same leaf in moist chamber. Numerous 

 acervuli developed in all, showing a tendency to form about cavities 

 in the agar where portions had been removed for subcultures. This 

 behavior was also shown in plates made from the orange, but was 

 not noticed in the other plates from lemon. 



252 



