STUDIES OF GLOMEEELLA FROM DIFFERENT HOSTS. 23 



the fungus in the tissues of both young and old leaves, but it is either 

 more widely distributed in the old leaves or else develops in them more 

 rapidly, as discoloration of the leaves and fructification of the fungus 

 appeared sooner in most cases on old leaves. 



On May 16 one of the young fruits about three-fourths of an inch 

 in diameter was taken from the branch just above the two young 

 leaves already referred to, the surface sterilized, and placed in a sterile 

 moist chamber. On May 27 acervuli of Glomerella were found on the 

 inside of the calyx and also on the fruit. 



On March 6 other apparently healthy leaves, both young and old, 

 were collected from a blossoming tree in the greenhouse, treated as 

 usual, and placed in sterile moist chambers. Eight days later the 

 1-year-old and 2-year-old leaves showed abundant acervuli develop- 

 ing along the midrib, as indicated in Plate IV. The very young leaves 

 in this instance showed no indication of the fungus. Ten days later 

 the 1-year-old and 2-year-old leaves showed a number of discolored 

 areas with perithecia. In this case the areas producing conidia and 

 those producing perithecia were separate but contiguous, the two 

 forms together almost covering the entire surfaces, as shown in Plate 

 V. The young leaves now began to show discolored areas but no 

 signs of fungous growth. On April 14 the youngest leaves were still 

 free from any sign of fungus. 



Other old leaves collected January 29 and treated as usual also 

 developed an abundance of acervuli and perithecia. 



CULTURES. 



On March 16 cultures were made from conidia found on a fruit of 

 pomelo received from Miami, Fla. Setae were abundant in the acervuli 

 on the fruit. The fungus grew rapidly, producing a smoky-colored 

 growth like that in some of the cultures from the orange. Large 

 acervuli were produced bearing a very few setae. No perithecia were 

 ever found in these cultures. 



On May 14 other cultures were made from a pomelo fruit from 

 Bonita, Cal. These cultures were made by transplanting portions of 

 discolored spots or so-called u tear stains" from the fruit. The sur- 

 face of the fruit was sterilized as usual with corrosive sublimate. The 

 resulting growth was apparently pure and produced an abundance of 

 acervuli and peritheciumlike bodies. These cultures were kept until 

 August 13, but no asci or spores were found. 



On June 17 corn-meal agar plates were poured, using conidia from 

 acervuli surrounded by ascogenous perithecia from a leaf in moist 

 chamber. These cultures all produced acervuli. They were kept 

 until July 22 but showed no perithecia. No setae or chlamydospores 

 were seen in these cultures. 



252 



