STUDIES OF GLOMEKELLA FEOM DIFFERENT HOSTS. 19 



appeared in these plates and very dark setse were present in abund- 

 ance. The setse, however, were dark only at the apex and light col- 

 ored at the base, which is not the case with the setae in most other 

 forms. They also appeared to be somewhat shorter than usual. No 

 perithecia or peritheciumlike bodies appeared in the cultures. 



The fungus agrees with Gloeosporium lagenarium (Pass.) Sacc. and 

 Roum. . It seems probable that the fungus occurring on cucurbits 

 is specifically distinct from the other species investigated, though 

 Halsted (40) reports the successful transfer of the organism from 

 bean and pear to citron, Gitrullus vulgaris var. He also reports the 

 successful transfer of the organism from watermelon to bean, and 

 as a result reduces Colletotrichum UndemutJiianum to synonomy, using 

 Colletotrichum lagenarium (Pass.) E. and PL, which is the older name, 

 for anthracnose of bean as well as that of cucurbits. Evans (36) 

 states that the fungus passes from bean to watermelon and vice versa, 

 but the statement is apparently based upon Halsted's work (39) 

 and not on his own experiments. Further cultures and the oppor- 

 tunity to study the perithecial form from watermelon may be neces- 

 sary to determine this point satisfactorily. The experiments of 

 Edgerton (30) and the present writers in attempting to transfer the 

 organism from bean to cucurbits, as reported later, were all failures. 

 The writers' inoculations with the form from grape and guava to 

 watermelon, however, were entirely successful, but not conclusive as 

 to the identity of Colletotriclium lagenarium and Glomerella cingulata, 

 since the perithecial stage of the former is unknown. 



CITRUS AURANTIUM SINENSIS L. (SWEET ORANGE). 



Glomerella cingulata (Stonem.) S. and v. S. 

 Colletotrichum gloeosporioides Penz. 



DEVELOPMENT ON LEAVES AND BRANCHES IN MOIST CHAMBER. 



Since the fungus appeared to be present in a dormant or hiber- 

 nating condition in the tissues of the leaves, as indicated by its 

 appearance on apparently healthy leaves when their surfaces were 

 thoroughly sterilized and placed in sterile moist chamber, several 

 attempts were made to secure data which might throw some light 

 upon the location of the fungus in the tissues and its original point 

 of entrance. On March 6 apparently healthy leaves of various ages 

 were taken from a blossoming tree in the greenhouse. These were 

 treated as usual and placed in sterile moist chambers. On March 14 

 numerous acervuli were present on all the leaves except the youngest, 

 which showed a number of discolored spots but no other external evi- 

 dence of fungous infection. The acervuli on the older leaves usually 

 appeared first at the base of the midrib, being preceded by a dark, 

 water-soaked appearance of the tissue. In some cases the diseased 



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