STUDIES OF GLOMERELLA FROM DIFFERENT HOSTS. 17 



moist chamber and also in pure cultures. This matter is arranged 

 alphabetically with reference to the host plants. Following the 

 name of the host in each case is the name which has been adopted 

 for the organism, with synonyms also where they have been satis- 

 factorily determined. 



ANNONA CHERIMOLA MILL. (CHERIMOYA). 



Gloeosporium rufomaculans (Berk.) Thum. 



Poured plates of conidia from an acervulus on a stem of cherimoya 

 received from Miami, Fla., were made on April 9. Growth was 

 rapid. The mycelium was white at first, changing later to dark 

 greenish or smoke color and forming circular spots. Subcultures on 

 corn-meal agar in tubes produced an abundant growth of mycelium 

 and acervuli with a few setae. Later many setse appeared in these 

 cultures. Though the cultures were kept growing until January 19 

 of the following year, no perithecia were ever found. The fungus 

 from this host does not appear to have received a specific name. 

 The characters of the acervuli and the shape and measurements of 

 the spores averaged about the same as those from the apple, grape, 

 and citrus fruits. 



BRYA EBENUS (l.) DC. (JAMAICA EBONY). 



Glomerella cingulata (Stonem.) S. and v. S. 



Several leaves of the host plant, collected in the greenhouse on 

 January 19, were placed in sterile moist chamber. On February 8 

 acervuli of Gloeosporium were present on several of the leaves. 

 On February 17 many mature perithecia were found associated with 

 the acervuli. An ascus and ascospores are shown in Plate II, figures 

 23 and 23 a. The fungus showed no characters by which it could 

 be distinguished from the Glomerella on apple and grape. No fungus 

 of this kind appears to have been heretofore reported upon this host. 



Plates were poured February 8, using conidia from a leaf taken 

 from an apparently healthy plant in the Department greenhouse. 

 The acervuli from which these cultures were made developed upon 

 this leaf in a sterile moist chamber. Spores germinated quickly and 

 subcultures in tubes of corn-meal agar were made by transferring 

 single conidia. Growth in the plates was similar to that usually 

 produced by Gloeosporium from apple or grape. Conidia were pro- 

 duced in both plates and tubes, but no very distinct acervuli were 

 found. On February 17 the cultures were found to be contaminated 

 with a mold and had to be discarded. Perithecia were not found 

 in them. The cultures were perhaps not old enough at the time for 

 perithecia to have had an opportunity to develop. 

 46023°— Bui. 252—13 2 



