76 PAEASITES BELONGING TO THE GENUS GLOMERELLA. 



reports in the same place successful inoculations with ascospores 

 inserted in punctures, and he succeeded in infecting green fruit picked 

 from the trees by placing conidia in drops of water on the unpunc- 

 tured skin. In two experiments, however, no infection followed 

 spraying the spores on the surface of green fruit. In one experiment 

 he reports successful infection by applying ascospores to the unbroken 

 surface in a drop of water. 



Von Schrenk and Spaulding (70) report the successful inoculation of 

 apple limbs by the introduction of ascospores in slits in the bark. 

 They also report successful infections on healthy fruit with spores 

 inserted in punctures and one successful experiment in infecting picked 

 fruit in a moist chamber by spores applied to the unbroken skin. 

 Scott (73) also has made successful inoculations of apples with conidia 

 without puncture. 



It will be noted that very few cases have been reported of successful 

 infection of apples without punctures, and much more information is 

 needed in regard to the exact time and method by which infection of 

 the apple takes place. The occurrence of conidia-producing cankers 

 on branches is too rare in most cases, in the East at least, to account 

 for the great prevalence and immense number of the infections which 

 are found during favorable seasons. 



APPLE TO BEAN. 



Halsted (40) reports successful inoculation of bean pods from 

 apple by the introduction of a portion of the decayed tissue or spores. 

 This is the only successful inoculation of beans with the apple anthrac- 

 nose that has been reported, and in view of the failure of other inves- 

 tigators to secure the same results it does not seem advisable without 

 verification to give it much weight in determining the specific rela- 

 tionship between the two forms occurring on these hosts. 



APPLE TO GRAPE. 



On October 14 eight berries of mature Niagara grapes were inocu- 

 lated by puncture with conidia from apples. Eight others were 

 inoculated by puncture with conidia from a culture. At the end of 

 two weeks the first eight all showed rot and acervuli had formed on 

 five berries. At the end of the same period the other lot all showed 

 rot, but only two bore acervuli. 



APPLE TO PUMPKIN. 



One mature pumpkin was inoculated by puncture with conidia of 

 Glomerella from an apple. No definite result was obtained, as the 

 pumpkin soon decayed from other causes. 



252 



