INOCULATION EXPERIMENTS. 77 



AVOCADO TO APPLE. 



On December 17 three apples were inoculated by puncture, using 

 conidia from a fruit of avocado. At the end of the week all showed 

 decayed spots 1 inch in diameter at the point of inoculation with 

 acervuli present in two cases. The other spot was smaller with no 

 acervuli present at first, but many developed a little later. 



AVOCADO TO BEAN. 



On June 4 six young pods of wax bean were inoculated with conidia. 

 No signs of infection ever appeared. 



AVOCADO TO CABBAGE. 



On November 8 young cabbage plants, bearing 8 to 10 leaves, were 

 inoculated with conidia from a pure culture. Inoculations were 

 made bj puncture and by surface application. No signs of infection 

 ever appeared on the inoculated leaves. 



AVOCADO TO COTTON. 



On April 1 three open cotton flowers were inoculated by applying 

 conidia in a drop of sterile water to the stigma of the flower. The 

 flowers were then covered with paper bags. No signs of infection had 

 appeared at the end of two weeks, and no conidia developed on the 

 bolls when removed and placed in moist chamber. 



On November 8 bolls one-half grown were inoculated with conidia 

 from a pure culture. Part of the inoculations were made on the 

 surface, the others by puncture. No signs of infection appeared 

 at the end of a month. 



AVOCADO TO RUBBER PLANT. 



Leaves were inoculated with conidia from pure culture, some 

 applied to the surface and some by punctures. No signs of infection 

 followed in either case. 



AVOCADO TO TEA. 



On November 8 young leaves were inoculated by both surface and 

 puncture methods with conidia from a pure culture. No signs of 

 infection appeared at the end of a month. 



BEAN TO APPLE. 



On September 30 four apples were inoculated by puncture with 

 conidia from a bean pod. No signs of infection had appeared at the 

 end of a month. 



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