INOCULATION EXPERIMENTS. 83 



FIG TO APPLE. 



On September 14 four apples inoculated by puncture with conidia 

 from a pure culture from the fig produced decay of the usual appear- 

 ance of bitter rot, and acervuli were formed. 



On October 2 three apples were inoculated by puncture with 

 conidia from a pure culture from the fig. Infection followed in all 

 cases and decay developed as rapidly as in inoculations made directly 

 from apple to apple and typical acervuli also developed as shown 

 in Plate XVI, figure 7. 



FIG TO FIG. 



Eight nearly mature figs were inoculated, four by puncture with 

 conidia from cultures and four by application of the same to the 

 unbroken surface of the fruit. Rot developed in all cases and 

 acervuli formed. There is some doubt about this experiment, 

 however, on account of the fact that three out of the seven checks 

 used also developed rot. 



FIG TO GRAPE. 



Eight mature berries were inoculated by puncture with conidia 

 from a pure culture from the fig. Most of the berries were rotten 

 at the end of three weeks and three showed acervuli. 



FICUS LONGIFOLIA TO RUBBER PLANT. 



The under side of a young leaf attached to a plant was inoculated 

 by placing conidia from a pure culture in sterile water on the unin- 

 jured surface and covering the spot with a Van Tiegham cell. At 

 the end of 24 days there was no sign of infection. The leaf was then 

 removed from the plant and placed in a moist chamber. Acervuli 

 developed on the inoculated spot after two weeks but not on the 

 check spot covered in the same way. 



GOOSEBERRY TO APPLE. 



On December 7 three apples were inoculated by puncture using 

 hyphaB and probably ascospores from a pure culture containing 

 masses of mature perithecia. On December 15 two of the apples 

 showed rotten spots 1 cm. in diameter at the points of inoculation 

 and the third a discoloration of the skin. By January 7 the first 

 two apples were half rotten and the third about one-third decaj^ed, 

 and two of them produced acervuli. Cultures made from these 

 apples produced both acervuli and perithecia of the fungus, the 

 perithecia predominating. 



252 



